PDB entry 4j88

View 4j88 on RCSB PDB site
Description: Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66
Class: fluorescent protein
Keywords: beta-barrel, fluorescent protein, Chromophore by cyclisation, p-Azido-L-Phenylalanine, cytosol
Deposited on 2013-02-14, released 2013-06-26
The last revision prior to the SCOPe 2.06 freeze date was dated 2013-06-26, with a file datestamp of 2013-06-21.
Experiment type: XRAY
Resolution: 2.08 Å
R-factor: 0.168
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (2-End)
      • engineered mutation (30)
      • engineered mutation (39)
      • see remark 999 (65)
      • see remark 999 (64)
      • engineered mutation (78)
      • engineered mutation (97)
      • engineered mutation (103)
      • engineered mutation (143)
      • engineered mutation (151)
      • engineered mutation (161)
      • engineered mutation (169)
      • engineered mutation (204)
    Domains in SCOPe 2.06: d4j88a_
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212
      • engineered mutation (30)
      • engineered mutation (39)
      • see remark 999 (65)
      • see remark 999 (64)
      • engineered mutation (78)
      • engineered mutation (97)
      • engineered mutation (103)
      • engineered mutation (143)
      • engineered mutation (151)
      • engineered mutation (161)
      • engineered mutation (169)
      • engineered mutation (204)
    Domains in SCOPe 2.06: d4j88b_
  • Heterogens: SO4, EDO, TRS, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4j88A (A:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlxvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvn
rielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqladh
yqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelykgsh
hhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4j88A (A:)
skgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlv
ttlxvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnri
elkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqladhyq
qntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagith

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4j88B (B:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlxvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvn
rielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqladh
yqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelykgsh
hhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4j88B (B:)
kgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlvt
tlxvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnrie
lkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqladhyqq
ntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagith