PDB entry 4iud

View 4iud on RCSB PDB site
Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form with ascorbate - partly reduced state
Class: oxidoreductase
Keywords: [NiFe] hydrogenase, Knallgas bacteria, proteobacteria, aerobic hydrogen bacteria, hydrogen catalysis, metalloenzyme, metalloprotein catalytic center, nickel-iron cofactor, bimetallic, Ni-Fe active site, iron-sulfur cluster, [4Fe-3S] cluster, [3Fe-4S] cluster, [4Fe-4S] cluster, reduced state, oxidized state, oxygen-tolerant hydrogenase, membrane-bound, OXIDOREDUCTASE
Deposited on 2013-01-20, released 2014-04-02
The last revision prior to the SCOPe 2.05 freeze date was dated 2014-05-14, with a file datestamp of 2014-05-09.
Experiment type: XRAY
Resolution: 1.45 Å
R-factor: 0.136
AEROSPACI score: 0.62 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'L':
    Compound: Uptake hydrogenase large subunit
    Species: Ralstonia eutropha [TaxId:381666]
    Gene: hoxG, PHG002
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.05: d4iudl_
  • Chain 'S':
    Compound: Uptake hydrogenase small subunit
    Species: Ralstonia eutropha [TaxId:381666]
    Gene: hoxK, PHG001
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.05: d4iuds_
  • Heterogens: NFV, MG, SF4, F3S, S3F, CL, HOH

PDB Chain Sequences:

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >4iudL (L:)
msayatqgfnlddrgrrivvdpvtrieghmrcevnvdannvirnavstgtmwrglevilk
grdprdawafvericgvctgchalasvravenaldiripknahlireimaktlqvhdhav
hfyhlhaldwvdvmsalkadpkrtselqqlvspahplssagyfrdiqnrlkrfvesgqlg
pfmngywgskayvlppeanlmavthylealdlqkewvkihtifggknphpnylvggvpca
inldgigaasapvnmerlsfvkarideiiefnknvyvpdvlaigtlykqagwlyggglaa
tnvldygeypnvaynkstdqlpggailngnwdevfpvdprdsqqvqefvshswykyades
vglhpwdgvtepnyvlgantkgtrtrieqidesakyswiksprwrghamevgplsryila
yaharsgnkyaerpkeqleysaqminsaipkalglpetqytlkqllpstigrtlarales
qycgemmhsdwhdlvaniragdtatanvdkwdpatwplqakgvgtvaaprgalghwirik
dgrienyqcvvpttwngsprdykgqigafeaslmntpmvnpeqpveilrtlhsfdpclac
sth

    Sequence, based on observed residues (ATOM records): (download) 
    >4iudL (L:)
ayatqgfnlddrgrrivvdpvtrieghmrcevnvdannvirnavstgtmwrglevilkgr
dprdawafvericgvctgchalasvravenaldiripknahlireimaktlqvhdhavhf
yhlhaldwvdvmsalkadpkrtselqqlvspahplssagyfrdiqnrlkrfvesgqlgpf
mngywgskayvlppeanlmavthylealdlqkewvkihtifggknphpnylvggvpcain
lsapvnmerlsfvkarideiiefnknvyvpdvlaigtlykqagwlyggglaatnvldyge
ypnvaynkstdqlpggailngnwdevfpvdprdsqqvqefvshswykyadesvglhpwdg
vtepnyvlgantkgtrtrieqidesakyswiksprwrghamevgplsryilayaharsgn
kyaerpkeqleysaqminsaipkalglpetqytlkqllpstigrtlaralesqycgemmh
sdwhdlvaniragdtatanvdkwdpatwplqakgvgtvaaprgalghwirikdgrienyq
cvvpttwngsprdykgqigafeaslmntpmvnpeqpveilrtlhsfdpclacsth

  • Chain 'S':
    Sequence, based on SEQRES records: (download) 
    >4iudS (S:)
metkprtpvlwlhglectccsesfirsahplakdvvlsmisldyddtlmaaaghqaeail
eeimtkykgnyilavegnpplnqdgmsciiggrpfieqlkyvakdakaiiswgscaswgc
vqaakpnptqatpvhkvitdkpiikvpgcppiaevmtgvitymltfdripeldrqgrpkm
fysqrihdkcyrrphfdagqfveewddesarkgfclykmgckgpttynacsttrwnegts
fpiqsghgcigcsedgfwdkgsfydrltgisqfgveanadkiggtasvvvgaavtahaaa
saikraskknetsgsehrsawshpqfekrsawshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >4iudS (S:)
prtpvlwlhglectccsesfirsahplakdvvlsmisldyddtlmaaaghqaeaileeim
tkykgnyilavegnpplnqdgmsciiggrpfieqlkyvakdakaiiswgscaswgcvqaa
kpnptqatpvhkvitdkpiikvpgcppiaevmtgvitymltfdripeldrqgrpkmfysq
rihdkcyrrphfdagqfveewddesarkgfclykmgckgpttynacsttrwnegtsfpiq
sghgcigcsedgfwdkgsfydrltg