PDB entry 4it1

View 4it1 on RCSB PDB site
Description: Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate
Class: isomerase
Keywords: isomerase, dehydrogenase, magnesium binding nadp, structural genomics, enzyme function initiative, efi
Deposited on 2013-01-17, released 2013-01-30
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-01-24, with a file datestamp of 2018-01-19.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Putative glucarate dehydratase
    Species: Pseudomonas fluorescens [TaxId:205922]
    Gene: Pfl01_3283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3KB33 (22-445)
      • expression tag (20-21)
    Domains in SCOPe 2.08: d4it1a1, d4it1a2, d4it1a3
  • Chain 'B':
    Compound: Putative glucarate dehydratase
    Species: Pseudomonas fluorescens [TaxId:205922]
    Gene: Pfl01_3283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3KB33 (22-445)
      • expression tag (21)
    Domains in SCOPe 2.08: d4it1b1, d4it1b2, d4it1b3
  • Chain 'C':
    Compound: Putative glucarate dehydratase
    Species: Pseudomonas fluorescens [TaxId:205922]
    Gene: Pfl01_3283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3KB33 (22-445)
      • expression tag (20-21)
    Domains in SCOPe 2.08: d4it1c1, d4it1c2, d4it1c3
  • Chain 'D':
    Compound: Putative glucarate dehydratase
    Species: Pseudomonas fluorescens [TaxId:205922]
    Gene: Pfl01_3283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3KB33 (22-445)
      • expression tag (19-21)
    Domains in SCOPe 2.08: d4it1d1, d4it1d2, d4it1d3
  • Heterogens: MG, TLA, K, BCT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4it1A (A:)
mhhhhhhssgvdlgtenlyfqsmkitrvtvtpiafrdppllnasgihepfalrsiieies
dngyiglgesygdapalaiqqqvqsqligldpfnlnqlrrivqttvaahkpaslagaela
pgshaskavsnaysafevafldlqarylnvplvdllggavrdevpfsaylffkyaqhvds
pykpdnwgealneqqivaqaarmieaygfksiklkagtlppehevacikalkkafpgypl
ridpngnwsletsirmaellgddlqyyedptpglegmaelhkrtglplatnmvvtdfdef
rrsvaqnsvqivladhhywgglrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaa
vpnldyacdthypwqepdeevikggklpivdgcvkitrapglgleldhdqlgklhdqylt
cgirqrddvrqmqrykpdwkalkprf

    Sequence, based on observed residues (ATOM records): (download) 
    >4it1A (A:)
qsmkitrvtvtpiafrdppllnasgihepfalrsiieiesdngyiglgesygdapalaiq
qqvqsqligldpfnlnqlrrivqttvaahkpaslagaelapgshaskavsnaysafevaf
ldlqarylnvplvdllggavrdevpfsaylffkyaqhvdspykpdnwgealneqqivaqa
armieaygfksiklkagtlppehevacikalkkafpgyplridpngnwsletsirmaell
gddlqyyedptpglegmaelhkrtglplatnmvvtdfdefrrsvaqnsvqivladhhywg
glrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaavpnldyacdthypwqepdee
vikggklpivdgcvkitrapglgleldhdqlgklhdqyltcgirqrddvrqmqrykpdwk
alkprf

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4it1B (B:)
mhhhhhhssgvdlgtenlyfqsmkitrvtvtpiafrdppllnasgihepfalrsiieies
dngyiglgesygdapalaiqqqvqsqligldpfnlnqlrrivqttvaahkpaslagaela
pgshaskavsnaysafevafldlqarylnvplvdllggavrdevpfsaylffkyaqhvds
pykpdnwgealneqqivaqaarmieaygfksiklkagtlppehevacikalkkafpgypl
ridpngnwsletsirmaellgddlqyyedptpglegmaelhkrtglplatnmvvtdfdef
rrsvaqnsvqivladhhywgglrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaa
vpnldyacdthypwqepdeevikggklpivdgcvkitrapglgleldhdqlgklhdqylt
cgirqrddvrqmqrykpdwkalkprf

    Sequence, based on observed residues (ATOM records): (download) 
    >4it1B (B:)
smkitrvtvtpiafrdppllnasgihepfalrsiieiesdngyiglgesygdapalaiqq
qvqsqligldpfnlnqlrrivqttvaahkpaslagaelapgshaskavsnaysafevafl
dlqarylnvplvdllggavrdevpfsaylffkyaqhvdspykpdnwgealneqqivaqaa
rmieaygfksiklkagtlppehevacikalkkafpgyplridpngnwsletsirmaellg
ddlqyyedptpglegmaelhkrtglplatnmvvtdfdefrrsvaqnsvqivladhhywgg
lrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaavpnldyacdthypwqepdeev
ikggklpivdgcvkitrapglgleldhdqlgklhdqyltcgirqrddvrqmqrykpdwka
lkprf

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4it1C (C:)
mhhhhhhssgvdlgtenlyfqsmkitrvtvtpiafrdppllnasgihepfalrsiieies
dngyiglgesygdapalaiqqqvqsqligldpfnlnqlrrivqttvaahkpaslagaela
pgshaskavsnaysafevafldlqarylnvplvdllggavrdevpfsaylffkyaqhvds
pykpdnwgealneqqivaqaarmieaygfksiklkagtlppehevacikalkkafpgypl
ridpngnwsletsirmaellgddlqyyedptpglegmaelhkrtglplatnmvvtdfdef
rrsvaqnsvqivladhhywgglrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaa
vpnldyacdthypwqepdeevikggklpivdgcvkitrapglgleldhdqlgklhdqylt
cgirqrddvrqmqrykpdwkalkprf

    Sequence, based on observed residues (ATOM records): (download) 
    >4it1C (C:)
qsmkitrvtvtpiafrdppllnasgihepfalrsiieiesdngyiglgesygdapalaiq
qqvqsqligldpfnlnqlrrivqttvaahkpaslagaelapgshaskavsnaysafevaf
ldlqarylnvplvdllggavrdevpfsaylffkyaqhvdspykpdnwgealneqqivaqa
armieaygfksiklkagtlppehevacikalkkafpgyplridpngnwsletsirmaell
gddlqyyedptpglegmaelhkrtglplatnmvvtdfdefrrsvaqnsvqivladhhywg
glrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaavpnldyacdthypwqepdee
vikggklpivdgcvkitrapglgleldhdqlgklhdqyltcgirqrddvrqmqrykpdwk
alkprf

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4it1D (D:)
mhhhhhhssgvdlgtenlyfqsmkitrvtvtpiafrdppllnasgihepfalrsiieies
dngyiglgesygdapalaiqqqvqsqligldpfnlnqlrrivqttvaahkpaslagaela
pgshaskavsnaysafevafldlqarylnvplvdllggavrdevpfsaylffkyaqhvds
pykpdnwgealneqqivaqaarmieaygfksiklkagtlppehevacikalkkafpgypl
ridpngnwsletsirmaellgddlqyyedptpglegmaelhkrtglplatnmvvtdfdef
rrsvaqnsvqivladhhywgglrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaa
vpnldyacdthypwqepdeevikggklpivdgcvkitrapglgleldhdqlgklhdqylt
cgirqrddvrqmqrykpdwkalkprf

    Sequence, based on observed residues (ATOM records): (download) 
    >4it1D (D:)
fqsmkitrvtvtpiafrdppllnasgihepfalrsiieiesdngyiglgesygdapalai
qqqvqsqligldpfnlnqlrrivqttvaahkpaslagaelapgshaskavsnaysafeva
fldlqarylnvplvdllggavrdevpfsaylffkyaqhvdspykpdnwgealneqqivaq
aarmieaygfksiklkagtlppehevacikalkkafpgyplridpngnwsletsirmael
lgddlqyyedptpglegmaelhkrtglplatnmvvtdfdefrrsvaqnsvqivladhhyw
gglrdtqtlakmcdtfglgvsmhsnshlgislmamahvaaavpnldyacdthypwqepde
evikggklpivdgcvkitrapglgleldhdqlgklhdqyltcgirqrddvrqmqrykpdw
kalkprf