PDB entry 4hna

View 4hna on RCSB PDB site
Description: Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN
Class: motor protein
Keywords: alpha-tubulin, beta-tubulin, DARPin, GTPase, kinesin, microtubule, tubulin, MOTOR PROTEIN
Deposited on 2012-10-19, released 2013-06-12
The last revision prior to the SCOPe 2.04 freeze date was dated 2013-08-21, with a file datestamp of 2013-08-16.
Experiment type: XRAY
Resolution: 3.19 Å
R-factor: 0.178
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tubulin alpha chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • Uniprot D0VWZ0 (0-End)
      • see remark 999 (231)
      • see remark 999 (339)
    Domains in SCOPe 2.04: d4hnaa1, d4hnaa2
  • Chain 'B':
    Compound: Tubulin beta chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • Uniprot D0VWY9 (0-End)
      • see remark 999 (315)
    Domains in SCOPe 2.04: d4hnab1, d4hnab2
  • Chain 'D':
    Compound: Designed ankyrin repeat protein (DARPIN) D2
    Species: artificial gene [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 4HNA (Start-168)
    Domains in SCOPe 2.04: d4hnad_
  • Chain 'K':
    Compound: kinesin-1 heavy chain
    Species: Homo sapiens [TaxId:9606]
    Gene: KIF5B, KNS, KNS1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33176
      • see remark 999 (6)
      • see remark 999 (64)
      • see remark 999 (167)
      • see remark 999 (173)
      • see remark 999 (293)
      • see remark 999 (329)
    Domains in SCOPe 2.04: d4hnak_
  • Heterogens: GTP, MG, GDP, ALF, ADP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4hnaA (A:)
mrecisihvgqagvqignacwelyclehgiqpdgqmpsdktigggddsfntffsetgagk
hvpravfvdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvld
rirkladqctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvsta
vvepynsiltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssita
slrfdgalnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepan
qmvkcdprhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpp
tvvpggdlakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefse
aredmaalekdyeevgvdsvegegeeegeey

    Sequence, based on observed residues (ATOM records): (download) 
    >4hnaA (A:)
mrecisihvgqagvqignacwelyclehgiqpdgqmpsddsfntffsetgagkhvpravf
vdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvldrirklad
qctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvstavvepyns
iltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssitaslrfdga
lnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepanqmvkcdp
rhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpptvvpggd
lakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefsearedmaa
lekdyeevgv

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4hnaB (B:)
mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
prailvdlepgtmdsvrsgpfgqifrpdnfvfgqsgagnnwakghytegaelvdsvldvv
rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
epynatlsvhqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
aacdprhgryltvaaifrgrmsmkevdeqmlnvqnknssyfvewipnnvktavcdipprg
lkmsatfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
eyqqyqdatadeqgefeeeegedea

    Sequence, based on observed residues (ATOM records): (download) 
    >4hnaB (B:)
mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
prailvdlepgtmdsvrsgpfgqifrpdnfvfgqsgagnnwakghytegaelvdsvldvv
rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
epynatlsvhqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
aacdprhgryltvaaifrgrmsmkevdeqmlnvqnknssyfvewipnnvktavcdipprg
lkmsatfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
eyqqyqdatad

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4hnaD (D:)
mrgshhhhhhgsdlgkklleaaragqddevrilmangadvnaeddsgktplhlaaikghl
eivevllkhgadvnaadkmgdtplhlaalyghleivevllkngadvnatdtygftplhla
adaghleivevllkygadvnaqdkfgktafdisidngnedlaeilqkln

    Sequence, based on observed residues (ATOM records): (download) 
    >4hnaD (D:)
gsdlgkklleaaragqddevrilmangadvnaeddsgktplhlaaikghleivevllkhg
advnaadkmgdtplhlaalyghleivevllkngadvnatdtygftplhlaadaghleive
vllkygadvnaqdkfgktafdisidngnedlaeilqkln

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >4hnaK (K:)
madlaesnikvmcrfrplnesevnrgdkyiakfqgedtvviaskpyafdrvfqsstsqeq
vyndaakkivkdvlegyngtifaygqtssgkthtmegklhdpegmgiiprivqdifnyiy
smdenlefhikvsyfeiyldkirdlldvsktnlsvhedknrvpyvkgaterfvsspdevm
dtidegksnrhvavtnmnehssrshsiflinvkqentqteqklsgklylvdlagsekvsk
tgaegavldeakninkslsalgnvisalaegstyvpyrdskmtrilqdslggnarttivi
ccspssynesetkstllfgqraktikntvsvnveltaeqwkkkyekeke

    Sequence, based on observed residues (ATOM records): (download) 
    >4hnaK (K:)
aesnikvmcrfrplnesevnrgdkyiakfqgedtvviaskpyafdrvfqsstsqeqvynd
aakkivkdvlegyngtifaygqtssgkthtmegklhdpegmgiiprivqdifnyiysmde
nlefhikvsyfeiyldkirdlldvsktnlsvhedknrvpyvkgaterfvsspdevmdtid
egksnrhvavtnmnehssrshsiflinvkqentqteqklsgklylvdlagsekvsktgae
gavldeakninkslsalgnvisalaegstyvpyrdskmtrilqdslggnarttiviccsp
ssynesetkstllfgqraktikntvsvnvelta