PDB entry 4hna

View 4hna on RCSB PDB site
Description: Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN
Class: motor protein
Keywords: alpha-tubulin, beta-tubulin, DARPin, GTPase, kinesin, microtubule, tubulin, MOTOR PROTEIN
Deposited on 2012-10-19, released 2013-06-12
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-11-15, with a file datestamp of 2017-11-10.
Experiment type: XRAY
Resolution: 3.19 Å
R-factor: N/A
AEROSPACI score: 0.12 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tubulin alpha chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • Uniprot D0VWZ0 (0-End)
      • see remark 999 (231)
      • see remark 999 (339)
    Domains in SCOPe 2.07: d4hnaa1, d4hnaa2
  • Chain 'B':
    Compound: Tubulin beta chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • Uniprot D0VWY9 (0-End)
      • see remark 999 (315)
    Domains in SCOPe 2.07: d4hnab1, d4hnab2
  • Chain 'D':
    Compound: Designed ankyrin repeat protein (DARPIN) D2
    Species: artificial gene [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 4HNA (Start-168)
    Domains in SCOPe 2.07: d4hnad1, d4hnad2
  • Chain 'K':
    Compound: kinesin-1 heavy chain
    Species: Homo sapiens [TaxId:9606]
    Gene: KIF5B, KNS, KNS1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33176
      • see remark 999 (6)
      • see remark 999 (64)
      • see remark 999 (167)
      • see remark 999 (173)
      • see remark 999 (293)
      • see remark 999 (329)
    Domains in SCOPe 2.07: d4hnak_
  • Heterogens: GTP, MG, GDP, ALF, ADP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4hnaA (A:)
    mrecisihvgqagvqignacwelyclehgiqpdgqmpsdktigggddsfntffsetgagk
    hvpravfvdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvld
    rirkladqctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvsta
    vvepynsiltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssita
    slrfdgalnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepan
    qmvkcdprhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpp
    tvvpggdlakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefse
    aredmaalekdyeevgvdsvegegeeegeey
    

    Sequence, based on observed residues (ATOM records): (download)
    >4hnaA (A:)
    mrecisihvgqagvqignacwelyclehgiqpdgqmpsddsfntffsetgagkhvpravf
    vdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvldrirklad
    qctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvstavvepyns
    iltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssitaslrfdga
    lnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepanqmvkcdp
    rhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpptvvpggd
    lakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefsearedmaa
    lekdyeevgv
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >4hnaB (B:)
    mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
    prailvdlepgtmdsvrsgpfgqifrpdnfvfgqsgagnnwakghytegaelvdsvldvv
    rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
    epynatlsvhqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
    rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
    aacdprhgryltvaaifrgrmsmkevdeqmlnvqnknssyfvewipnnvktavcdipprg
    lkmsatfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
    eyqqyqdatadeqgefeeeegedea
    

    Sequence, based on observed residues (ATOM records): (download)
    >4hnaB (B:)
    mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
    prailvdlepgtmdsvrsgpfgqifrpdnfvfgqsgagnnwakghytegaelvdsvldvv
    rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
    epynatlsvhqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
    rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
    aacdprhgryltvaaifrgrmsmkevdeqmlnvqnknssyfvewipnnvktavcdipprg
    lkmsatfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
    eyqqyqdatad
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >4hnaD (D:)
    mrgshhhhhhgsdlgkklleaaragqddevrilmangadvnaeddsgktplhlaaikghl
    eivevllkhgadvnaadkmgdtplhlaalyghleivevllkngadvnatdtygftplhla
    adaghleivevllkygadvnaqdkfgktafdisidngnedlaeilqkln
    

    Sequence, based on observed residues (ATOM records): (download)
    >4hnaD (D:)
    gsdlgkklleaaragqddevrilmangadvnaeddsgktplhlaaikghleivevllkhg
    advnaadkmgdtplhlaalyghleivevllkngadvnatdtygftplhlaadaghleive
    vllkygadvnaqdkfgktafdisidngnedlaeilqkln
    

  • Chain 'K':
    Sequence, based on SEQRES records: (download)
    >4hnaK (K:)
    madlaesnikvmcrfrplnesevnrgdkyiakfqgedtvviaskpyafdrvfqsstsqeq
    vyndaakkivkdvlegyngtifaygqtssgkthtmegklhdpegmgiiprivqdifnyiy
    smdenlefhikvsyfeiyldkirdlldvsktnlsvhedknrvpyvkgaterfvsspdevm
    dtidegksnrhvavtnmnehssrshsiflinvkqentqteqklsgklylvdlagsekvsk
    tgaegavldeakninkslsalgnvisalaegstyvpyrdskmtrilqdslggnarttivi
    ccspssynesetkstllfgqraktikntvsvnveltaeqwkkkyekeke
    

    Sequence, based on observed residues (ATOM records): (download)
    >4hnaK (K:)
    aesnikvmcrfrplnesevnrgdkyiakfqgedtvviaskpyafdrvfqsstsqeqvynd
    aakkivkdvlegyngtifaygqtssgkthtmegklhdpegmgiiprivqdifnyiysmde
    nlefhikvsyfeiyldkirdlldvsktnlsvhedknrvpyvkgaterfvsspdevmdtid
    egksnrhvavtnmnehssrshsiflinvkqentqteqklsgklylvdlagsekvsktgae
    gavldeakninkslsalgnvisalaegstyvpyrdskmtrilqdslggnarttiviccsp
    ssynesetkstllfgqraktikntvsvnvelta