PDB entry 4hn1
View 4hn1 on RCSB PDB site
Description: Crystal Structure of H60N/Y130F double mutant of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP
Class: unknown function
Keywords: 3'-monoepimerase, natural product, deoxysugar, chalcomycin, dTDP-mycinose, Cupin fold, nucleotide-linked sugar, Epimerization, UNKNOWN FUNCTION
Deposited on
2012-10-18, released
2012-11-21
The last revision prior to the SCOPe 2.08 freeze date was dated
2012-12-05, with a file datestamp of
2012-11-30.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: 0.214
AEROSPACI score: 0.54
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: Putative 3-epimerase in D-allose pathway
Species: Streptomyces bikiniensis [TaxId:1896]
Gene: chmJ
Database cross-references and differences (RAF-indexed):
- Uniprot Q5SFD1 (0-195)
- engineered mutation (59)
- engineered mutation (129)
- expression tag (196-198)
Domains in SCOPe 2.08: d4hn1a1, d4hn1a2 - Chain 'B':
Compound: Putative 3-epimerase in D-allose pathway
Species: Streptomyces bikiniensis [TaxId:1896]
Gene: chmJ
Database cross-references and differences (RAF-indexed):
- Uniprot Q5SFD1 (0-195)
- engineered mutation (59)
- engineered mutation (129)
- expression tag (196-198)
Domains in SCOPe 2.08: d4hn1b1, d4hn1b2 - Chain 'C':
Compound: Putative 3-epimerase in D-allose pathway
Species: Streptomyces bikiniensis [TaxId:1896]
Gene: chmJ
Database cross-references and differences (RAF-indexed):
- Uniprot Q5SFD1 (0-195)
- engineered mutation (59)
- engineered mutation (129)
- expression tag (196-200)
Domains in SCOPe 2.08: d4hn1c1, d4hn1c2 - Chain 'D':
Compound: Putative 3-epimerase in D-allose pathway
Species: Streptomyces bikiniensis [TaxId:1896]
Gene: chmJ
Database cross-references and differences (RAF-indexed):
- Uniprot Q5SFD1 (0-195)
- engineered mutation (59)
- engineered mutation (129)
- expression tag (196-199)
Domains in SCOPe 2.08: d4hn1d1, d4hn1d2 - Heterogens: EDO, TYD, THM, TDR, HOH
PDB Chain Sequences:
- Chain 'A':
Sequence, based on SEQRES records: (download)
>4hn1A (A:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrlehhh
Sequence, based on observed residues (ATOM records): (download)
>4hn1A (A:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrleh
- Chain 'B':
Sequence, based on SEQRES records: (download)
>4hn1B (B:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrlehhh
Sequence, based on observed residues (ATOM records): (download)
>4hn1B (B:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrleh
- Chain 'C':
Sequence; same for both SEQRES and ATOM records: (download)
>4hn1C (C:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrlehhh
- Chain 'D':
Sequence, based on SEQRES records: (download)
>4hn1D (D:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrlehhh
Sequence, based on observed residues (ATOM records): (download)
>4hn1D (D:)
mhplsiegawsqepvihsdhrgrshewfrgesfrqafghdfpvaqvnvavshrgalrgin
yteippgqakysvcvrgagldvvvdvrigsptfgrweivpmdaerntavyltaglgrafl
sltddatlvflcssgyaparehsvnpldpdlgiawpddiepllsdrdenaptlataerlg
llptyqawqeqqqaqrlehh