PDB entry 4fhb

View 4fhb on RCSB PDB site
Description: Enhancing DHFR catalysis by binding of an allosteric regulator nanobody (Nb179)
Class: Oxidoreductase/protein binding
Keywords: Nanobody, Dihydrofolate reductase, Allosteric activator, Reductase, Oxidoreductase-protein binding complex
Deposited on 2012-06-06, released 2013-04-24
The last revision prior to the SCOPe 2.06 freeze date was dated 2013-09-11, with a file datestamp of 2013-09-06.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.221
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: dihydrofolate reductase
    Species: Escherichia coli [TaxId:83333]
    Gene: folA, tmrA, b0048, JW0047
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d4fhba_
  • Chain 'D':
    Compound: Nb179
    Species: Lama glama [TaxId:9844]
    Database cross-references and differences (RAF-indexed):
    • PDB 4FHB
    Domains in SCOPe 2.06: d4fhbd1, d4fhbd2
  • Heterogens: FOL, NAP

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4fhbA (A:)
misliaalavdrvigmenampwnlpadlawfkrntlnkpvimgrhtwesigrplpgrkni
ilssqpgtddrvtwvksvdeaiaacgdvpeimvigggrvyeqflpkaqklylthidaeve
gdthfpdyepddwesvfsefhdadaqnshsycfeilerr

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4fhbD (D:)
qvqlqesggglvqaggslrlsceasgrtfssyamgwfrqapgkerdfvaviswsgsntyy
adsakgrftisrdnakntvylqmnslkpedtaiyycaapgrphgsswslnkkgqgydywg
qgtqvtvsshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4fhbD (D:)
qlqesggglvqaggslrlsceasgrtfssyamgwfrqaperdfvaviswsgsntyyadsa
kgrftisrdnakntvylqmnslkpedtaiyycaapgrphgsswslnkkgqgydywgqgtq
vtvss