PDB entry 4f5j

View 4f5j on RCSB PDB site
Description: Rational Design and Directed Evolution for Conversion of Substrate Specificity from E.coli Aspartate Aminotransferase to Tyrosine Aminotransferase: Mutant P5.
Class: Transferase
Keywords: Aminotransferase, Transferase
Deposited on 2012-05-13, released 2013-02-13
The last revision prior to the SCOPe 2.06 freeze date was dated 2013-04-24, with a file datestamp of 2013-04-19.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: 0.163
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: aspartate aminotransferase
    Species: Escherichia coli [TaxId:83333]
    Gene: AAT, aspC, b0928, JW0911
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00509 (11-405)
      • expression tag (7-10)
      • engineered mutation (38-39)
      • engineered mutation (42)
      • engineered mutation (73)
      • engineered mutation (77)
      • engineered mutation (80)
      • engineered mutation (113)
      • engineered mutation (144-145)
      • engineered mutation (196)
      • engineered mutation (219)
      • engineered mutation (221)
      • engineered mutation (227)
      • engineered mutation (253)
    Domains in SCOPe 2.06: d4f5ja1, d4f5ja2
  • Chain 'B':
    Compound: aspartate aminotransferase
    Species: Escherichia coli [TaxId:83333]
    Gene: AAT, aspC, b0928, JW0911
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00509 (11-405)
      • expression tag (7-10)
      • engineered mutation (38-39)
      • engineered mutation (42)
      • engineered mutation (73)
      • engineered mutation (77)
      • engineered mutation (80)
      • engineered mutation (113)
      • engineered mutation (144-145)
      • engineered mutation (196)
      • engineered mutation (219)
      • engineered mutation (221)
      • engineered mutation (227)
      • engineered mutation (253)
    Domains in SCOPe 2.06: d4f5jb1, d4f5jb2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4f5jA (A:)
mahhhhhhvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvltsvk
kaeqyllenettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrva
adflakntsvkrvwvsnpswpnhkaifnsaglevreyayydaenhtldfdalinslneaq
agdvvlfhgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglra
faamhkelivasscsknfglynervgactlvaadsetvdrafsqmkaairanysnppahg
asvvatilsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsf
sgltkeqvlrlreefgvyavasgrvnvagmtpdnmaplceaivavl

    Sequence, based on observed residues (ATOM records): (download) 
    >4f5jA (A:)
hvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvltsvkkaeqyll
enettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrvaadflakn
tsvkrvwvsnpswpnhkaifnsaglevreyayydaenhtldfdalinslneaqagdvvlf
hgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglrafaamhke
livasscsknfglynervgactlvaadsetvdrafsqmkaairanysnppahgasvvati
lsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsfsgltkeq
vlrlreefgvyavasgrvnvagmtpdnmaplceaivavl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4f5jB (B:)
mahhhhhhvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvltsvk
kaeqyllenettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrva
adflakntsvkrvwvsnpswpnhkaifnsaglevreyayydaenhtldfdalinslneaq
agdvvlfhgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglra
faamhkelivasscsknfglynervgactlvaadsetvdrafsqmkaairanysnppahg
asvvatilsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsf
sgltkeqvlrlreefgvyavasgrvnvagmtpdnmaplceaivavl

    Sequence, based on observed residues (ATOM records): (download) 
    >4f5jB (B:)
hvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvltsvkkaeqyll
enettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrvaadflakn
tsvkrvwvsnpswpnhkaifnsaglevreyayydaenhtldfdalinslneaqagdvvlf
hgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglrafaamhke
livasscsknfglynervgactlvaadsetvdrafsqmkaairanysnppahgasvvati
lsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsfsgltkeq
vlrlreefgvyavasgrvnvagmtpdnmaplceaivavl