PDB entry 4f4j

View 4f4j on RCSB PDB site
Description: Conversion of the enzyme guanylate kinase into a mitotic spindle orienting protein by a single mutation that inhibits gmp- induced closing
Class: transferase
Keywords: Phosphotransferase, GMP and ATP, TRANSFERASE
Deposited on 2012-05-10, released 2012-06-13
The last revision prior to the SCOPe 2.05 freeze date was dated 2012-06-13, with a file datestamp of 2012-06-08.
Experiment type: XRAY
Resolution: 2.45 Å
R-factor: 0.218
AEROSPACI score: 0.32 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanylate kinase
    Species: Saccharomyces cerevisiae [TaxId:559292]
    Gene: GUK1, YDR454C, D9461.39
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15454 (15-End)
      • expression tag (11-14)
      • engineered mutation (49)
    Domains in SCOPe 2.05: d4f4ja_
  • Chain 'B':
    Compound: Guanylate kinase
    Species: Saccharomyces cerevisiae [TaxId:559292]
    Gene: GUK1, YDR454C, D9461.39
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15454 (15-End)
      • expression tag (0-14)
      • engineered mutation (49)
    Domains in SCOPe 2.05: d4f4jb_
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4f4jA (A:)
    dydipttenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtprage
    vngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgv
    ksvkaipelnarflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdk
    vivnddldkaykelkdfifaek
    

    Sequence, based on observed residues (ATOM records): (download)
    >4f4jA (A:)
    fqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtpragevngkdynfvsv
    defksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgvksvkaipelna
    rflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdkvivnddldkay
    kelkdfifa
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >4f4jB (B:)
    dydipttenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtprage
    vngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgv
    ksvkaipelnarflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdk
    vivnddldkaykelkdfifaek
    

    Sequence, based on observed residues (ATOM records): (download)
    >4f4jB (B:)
    dydipttenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtprage
    vngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgv
    ksvkaipelnarflfiappsvedlkkkrlsaaqaelayaetgahdkvivnddldkaykel
    kdfifa