PDB entry 4epk

View 4epk on RCSB PDB site
Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase
Class: lyase
Keywords: ACMSD, tim-barrel, metaloenzyme, decarboxylase, LYASE
Deposited on 2012-04-17, released 2012-08-22
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-10-21, with a file datestamp of 2020-10-16.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: N/A
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase
    Species: Pseudomonas fluorescens [TaxId:294]
    Gene: nbaD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q83V25
      • engineered mutation (227)
    Domains in SCOPe 2.08: d4epka_
  • Chain 'B':
    Compound: 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase
    Species: Pseudomonas fluorescens [TaxId:294]
    Gene: nbaD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q83V25
      • engineered mutation (227)
    Domains in SCOPe 2.08: d4epkb_
  • Heterogens: ZN, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4epkA (A:)
mkkpridmhshffpriseqeaakfdanhapwlqvsakgdtgsimmgknnfrpvyqalwdp
afrieemdaqgvdvqvtcatpvmfgytweankaaqwaermndfalefaahnpqrikvlaq
vplqdldlackeasravaaghlgiqignhlgdkdlddatleaflthcanedipilvhpwd
mmggqrmkkwmlpwlvampaetqlailslilsgaferipkslkicfgggggsfafllgrv
dnawrhrdivredcprppseyvdrffvdsavfnpgalellvsvmgedrvmlgsdypfplg
eqkigglvlssnlgesakdkiisgnaskffninv

    Sequence, based on observed residues (ATOM records): (download) 
    >4epkA (A:)
kpridmhshffpriseqeaakfdanhapwlqvsakgdtgsimmgknnfrpvyqalwdpaf
rieemdaqgvdvqvtcatpvmfgytweankaaqwaermndfalefaahnpqrikvlaqvp
lqdldlackeasravaaghlgiqignhlgdkdlddatleaflthcanedipilvhpwdmm
ggqrmkkwmlpwlvampaetqlailslilsgaferipkslkicfgggggsfafllgrvdn
awrhrdivredcprppseyvdrffvdsavfnpgalellvsvmgedrvmlgsdypfplgeq
kigglvlssnlgesakdkiisgnaskffnin

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4epkB (B:)
mkkpridmhshffpriseqeaakfdanhapwlqvsakgdtgsimmgknnfrpvyqalwdp
afrieemdaqgvdvqvtcatpvmfgytweankaaqwaermndfalefaahnpqrikvlaq
vplqdldlackeasravaaghlgiqignhlgdkdlddatleaflthcanedipilvhpwd
mmggqrmkkwmlpwlvampaetqlailslilsgaferipkslkicfgggggsfafllgrv
dnawrhrdivredcprppseyvdrffvdsavfnpgalellvsvmgedrvmlgsdypfplg
eqkigglvlssnlgesakdkiisgnaskffninv

    Sequence, based on observed residues (ATOM records): (download) 
    >4epkB (B:)
pridmhshffpriseqeaakfdanhapwlqvsakgdtgsimmgknnfrpvyqalwdpafr
ieemdaqgvdvqvtcatpvmfgytweankaaqwaermndfalefaahnpqrikvlaqvpl
qdldlackeasravaaghlgiqignhlgdkdlddatleaflthcanedipilvhpwdmmg
gqrmkkwmlpwlvampaetqlailslilsgaferipkslkicfgggggsfafllgrvdna
wrhrdivredcprppseyvdrffvdsavfnpgalellvsvmgedrvmlgsdypfplgeqk
igglvlssnlgesakdkiisgnaskffnin