PDB entry 4en1

View 4en1 on RCSB PDB site
Description: The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein
Class: fluorescent protein
Keywords: GFP-like beta-barrel, FLUORESCENT PROTEIN
Deposited on 2012-04-12, released 2013-04-24
The last revision prior to the SCOPe 2.05 freeze date was dated 2013-07-17, with a file datestamp of 2013-07-12.
Experiment type: XRAY
Resolution: 1.62 Å
R-factor: 0.179
AEROSPACI score: 0.58 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (20-254)
      • expression tag (19)
      • engineered mutation (82)
      • engineered mutation (88)
      • engineered mutation (161-164)
      • engineered mutation (169)
      • engineered mutation (179)
      • engineered mutation (182-185)
      • engineered mutation (222)
      • engineered mutation (247)
    Domains in SCOPe 2.05: d4en1a_
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212
      • engineered mutation (82)
      • engineered mutation (88)
      • engineered mutation (161-164)
      • engineered mutation (169)
      • engineered mutation (179)
      • engineered mutation (182-185)
      • engineered mutation (222)
    Domains in SCOPe 2.05: d4en1b_
  • Heterogens: GOL, PEG, ACT, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4en1A (A:)
mrgshhhhhhglalpvatmvskgeelftgvvpilveldgdvnghkfsvsgegegdatygk
ltlkficttgklpvpwptlvttlxvqcfarypdhmkqhdffksampegyvqertiffkdd
gnyktraevkfegdtlvnrielkgidfkedgnilghkleynaihgnvyitadkqkngika
nfglncniedgsvqladhyqqntpigdgpvllpdnhylstqsklskdpnekrdhmvllef
vtaagitlgmdelyksglrsraqa

    Sequence, based on observed residues (ATOM records): (download) 
    >4en1A (A:)
vskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptl
vttlxvqcfarypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnr
ielkgidfkedgnilghkleynaihgnvyitadkqkngikanfglncniedgsvqladhy
qqntpigdgpvllpdnhylstqsklskdpnekrdhmvllefvtaagitlgmdelyk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4en1B (B:)
mrgshhhhhhglalpvatmvskgeelftgvvpilveldgdvnghkfsvsgegegdatygk
ltlkficttgklpvpwptlvttlxvqcfarypdhmkqhdffksampegyvqertiffkdd
gnyktraevkfegdtlvnrielkgidfkedgnilghkleynaihgnvyitadkqkngika
nfglncniedgsvqladhyqqntpigdgpvllpdnhylstqsklskdpnekrdhmvllef
vtaagitlgmdelyksglrsraqa

    Sequence, based on observed residues (ATOM records): (download) 
    >4en1B (B:)
kgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlvt
tlxvqcfarypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrie
lkgidfkedgnilghkleynaihgnvyitadkqkngikanfglncniedgsvqladhyqq
ntpigdgpvllpdnhylstqsklskdpnekrdhmvllefvtaagit