PDB entry 4e97

View 4e97 on RCSB PDB site
Description: T4 Lysozyme L99A/M102H with 2-Mercaptoethanol Bound
Class: hydrolase
Keywords: Hydrolase, Alkylation with 2-mercaptoethanol
Deposited on 2012-03-20, released 2012-09-05
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-10-17, with a file datestamp of 2012-10-12.
Experiment type: XRAY
Resolution: 1.3 Å
R-factor: 0.143
AEROSPACI score: 0.8 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lysozyme
    Species: Enterobacteria phage T4 [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (23-186)
      • expression tag (12-22)
      • engineered mutation (43)
      • engineered mutation (60)
      • engineered mutation (121)
      • engineered mutation (124)
      • engineered mutation (130)
      • engineered mutation (139)
      • engineered mutation (164)
      • engineered mutation (166)
    Domains in SCOPe 2.08: d4e97a1, d4e97a2
  • Chain 'B':
    Compound: lysozyme
    Species: Enterobacteria phage T4 [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (23-186)
      • expression tag (9-22)
      • engineered mutation (43)
      • engineered mutation (60)
      • engineered mutation (121)
      • engineered mutation (124)
      • engineered mutation (130)
      • engineered mutation (139)
      • engineered mutation (164)
      • engineered mutation (166)
    Domains in SCOPe 2.08: d4e97b1, d4e97b2
  • Heterogens: BME, SO4, HED, ACT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4e97A (A:)
mghhhhhhdydipttenlyfqgsmnifemlrideglrlkiykdcegyytigighlltksp
dlnaakseldkaigrncngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrrc
aainhvfqmgvtgvagftnvlrmlqqkrwdeaavnlaksrwynqcpdrakrvittfrtgt
wdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >4e97A (A:)
pttenlyfqgsmnifemlrideglrlkiykdcegyytigighlltkspdlnaakseldka
igrncngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainhvfqmgvt
gvagftnvlrmlqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayknl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4e97B (B:)
mghhhhhhdydipttenlyfqgsmnifemlrideglrlkiykdcegyytigighlltksp
dlnaakseldkaigrncngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrrc
aainhvfqmgvtgvagftnvlrmlqqkrwdeaavnlaksrwynqcpdrakrvittfrtgt
wdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >4e97B (B:)
ydipttenlyfqgsmnifemlrideglrlkiykdcegyytigighlltkspdlnaaksel
dkaigrncngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainhvfqm
gvtgvagftnvlrmlqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayknl