PDB entry 4cyr

View 4cyr on RCSB PDB site
Description: G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa
Class: hydrolase
Keywords: hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
Deposited on 2014-04-14, released 2015-04-29
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-04-29, with a file datestamp of 2015-04-24.
Experiment type: XRAY
Resolution: 1.72 Å
R-factor: N/A
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P51691
      • engineered mutation (21)
      • engineered mutation (49)
      • engineered mutation (133)
      • engineered mutation (336)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cyra_
  • Chain 'B':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P51691
      • engineered mutation (21)
      • engineered mutation (49)
      • engineered mutation (133)
      • engineered mutation (336)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cyrb_
  • Heterogens: PEG, SO4, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4cyrA (A:)
mskrpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >4cyrA (A:)
krpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvve

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4cyrB (B:)
mskrpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >4cyrB (B:)
krpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvve