PDB entry 4cxu

View 4cxu on RCSB PDB site
Description: G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate
Class: hydrolase
Keywords: hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
Deposited on 2014-04-08, released 2015-04-22
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-04-22, with a file datestamp of 2015-04-17.
Experiment type: XRAY
Resolution: 2.03 Å
R-factor: N/A
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot U6AIT1
      • engineered mutation (21)
      • engineered mutation (49)
      • engineered mutation (133)
      • engineered mutation (336)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cxua_
  • Chain 'B':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot U6AIT1
      • engineered mutation (21)
      • engineered mutation (49)
      • engineered mutation (133)
      • engineered mutation (336)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cxub_
  • Heterogens: CA, 62Y, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4cxuA (A:)
mskrpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >4cxuA (A:)
krpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiagigtmaealgyeghlnervvalpellreagyqtlmagkwhlglkpeqtphargfe
rsfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssdafgdkllq
ylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlarlkelglve
adveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylrrqgeldnt
fvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwygprwaqaatap
srlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrhpgkrwrgr
eiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapvgpatwqly
dlardpgeihdladsqpgklaeliehwkryvsdtgvv

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4cxuB (B:)
mskrpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiagigtmaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >4cxuB (B:)
krpnflvivaddlgfsdigtfggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiagigtmaealgyeghlnervvalpellreagyqtlmagkwhlglkpeqtphargfe
rsfallpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssdafgdkllq
ylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlarlkelglve
adveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylrrqgeldnt
fvlfmsdngaegalleafpkfgpdlldfldrhydnslenigransyvwygprwaqaatap
srlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrhpgkrwrgr
eiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapvgpatwqly
dlardpgeihdladsqpgklaeliehwkryvsdtgvv