PDB entry 4cxk

View 4cxk on RCSB PDB site
Description: G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa
Class: hydrolase
Keywords: catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily, hydrolase
Deposited on 2014-04-07, released 2015-04-22
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-04-22, with a file datestamp of 2015-04-17.
Experiment type: XRAY
Resolution: 1.86 Å
R-factor: N/A
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P51691
      • engineered mutation (47)
      • engineered mutation (49)
      • engineered mutation (68)
      • engineered mutation (71)
      • engineered mutation (336)
      • engineered mutation (351)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cxka_
  • Chain 'B':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P51691
      • engineered mutation (47)
      • engineered mutation (49)
      • engineered mutation (68)
      • engineered mutation (71)
      • engineered mutation (336)
      • engineered mutation (351)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d4cxkb_
  • Heterogens: CA, SO4, PEG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4cxkA (A:)
mskrpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhttsaxsptrsmllt
gtdhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspfler

    Sequence, based on observed residues (ATOM records): (download) 
    >4cxkA (A:)
krpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhttsaxsptrsmlltgt
dhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvv

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4cxkB (B:)
mskrpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhttsaxsptrsmllt
gtdhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspfler

    Sequence, based on observed residues (ATOM records): (download) 
    >4cxkB (B:)
krpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhttsaxsptrsmlltgt
dhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvv