PDB entry 4cpi

View 4cpi on RCSB PDB site
Description: streptavidin A86D mutant with love-hate ligand 4
Class: biotin binding protein
Keywords: biotin binding protein, avidin, biotin, strain, biotinylated, steric clash, strained, hindered, force, ligand series, affinity
Deposited on 2014-02-06, released 2014-08-06
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-10-01, with a file datestamp of 2014-09-26.
Experiment type: XRAY
Resolution: 1.54 Å
R-factor: 0.1583
AEROSPACI score: 0.55 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (73)
    Domains in SCOPe 2.07: d4cpia_
  • Chain 'B':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (73)
    Domains in SCOPe 2.07: d4cpib_
  • Chain 'C':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (73)
    Domains in SCOPe 2.07: d4cpic_
  • Chain 'D':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (73)
    Domains in SCOPe 2.07: d4cpid_
  • Heterogens: LH4, PEG, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4cpiA (A:)
    aeagitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
    lgwtvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
    vkpsaas
    

    Sequence, based on observed residues (ATOM records): (download)
    >4cpiA (A:)
    gitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgtalgw
    tvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvk
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >4cpiB (B:)
    aeagitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
    lgwtvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
    vkpsaas
    

    Sequence, based on observed residues (ATOM records): (download)
    >4cpiB (B:)
    agitgtwynqlgstfivtagadgaltgtyesnaesryvltgrydsapatdgsgtalgwtv
    awknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvkp
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >4cpiC (C:)
    aeagitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
    lgwtvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
    vkpsaas
    

    Sequence, based on observed residues (ATOM records): (download)
    >4cpiC (C:)
    agitgtwynqlgstfivtagadgaltgtyesnaesryvltgrydsapatdgsgtalgwtv
    awknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvk
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >4cpiD (D:)
    aeagitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
    lgwtvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
    vkpsaas
    

    Sequence, based on observed residues (ATOM records): (download)
    >4cpiD (D:)
    gitgtwynqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgtalgw
    tvawknnyrndhsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvkp