PDB entry 4apb

View 4apb on RCSB PDB site
Description: Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate
Class: hydrolase
Keywords: hydrolase, metabolism
Deposited on 2012-03-31, released 2012-04-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-07-18, with a file datestamp of 2012-07-13.
Experiment type: XRAY
Resolution: 1.94 Å
R-factor: 0.1488
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: fumarate hydratase class II
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Database cross-references and differences (RAF-indexed):
    • Uniprot O53446
      • engineered mutation (317)
    Domains in SCOPe 2.08: d4apba_
  • Chain 'B':
    Compound: fumarate hydratase class II
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Database cross-references and differences (RAF-indexed):
    • Uniprot O53446
      • engineered mutation (317)
    Domains in SCOPe 2.08: d4apbb_
  • Chain 'C':
    Compound: fumarate hydratase class II
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Database cross-references and differences (RAF-indexed):
    • Uniprot O53446
      • engineered mutation (317)
    Domains in SCOPe 2.08: d4apbc_
  • Chain 'D':
    Compound: fumarate hydratase class II
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Database cross-references and differences (RAF-indexed):
    • Uniprot O53446
      • engineered mutation (317)
    Domains in SCOPe 2.08: d4apbd_
  • Heterogens: FUM, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4apbA (A:)
gavdadsanyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkga
caqvnsdlgllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasia
akggvtlhpnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtv
vksgrthlmdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapdd
fgvrvvavlvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgs
gpltglaeiqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnv
yipmmarnilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyee
aaavakqalkerktirqtvidrgligdrlsiedldrrldvlamakaeqldsdrl

    Sequence, based on observed residues (ATOM records): (download) 
    >4apbA (A:)
nyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkgacaqvnsdl
gllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasiaakggvtlh
pnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtvvksgrthl
mdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapddfgvrvvav
lvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgsgpltglae
iqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnvyipmmarn
ilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyeeaaavakqa
lkerktirqtvidrgligdrlsiedldrrldvlamakaeq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4apbB (B:)
gavdadsanyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkga
caqvnsdlgllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasia
akggvtlhpnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtv
vksgrthlmdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapdd
fgvrvvavlvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgs
gpltglaeiqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnv
yipmmarnilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyee
aaavakqalkerktirqtvidrgligdrlsiedldrrldvlamakaeqldsdrl

    Sequence, based on observed residues (ATOM records): (download) 
    >4apbB (B:)
nyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkgacaqvnsdl
gllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasiaakggvtlh
pnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtvvksgrthl
mdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapddfgvrvvav
lvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgsgpltglae
iqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnvyipmmarn
ilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyeeaaavakqa
lkerktirqtvidrgligdrlsiedldrrldvlamakaeq

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4apbC (C:)
gavdadsanyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkga
caqvnsdlgllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasia
akggvtlhpnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtv
vksgrthlmdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapdd
fgvrvvavlvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgs
gpltglaeiqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnv
yipmmarnilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyee
aaavakqalkerktirqtvidrgligdrlsiedldrrldvlamakaeqldsdrl

    Sequence, based on observed residues (ATOM records): (download) 
    >4apbC (C:)
nyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkgacaqvnsdl
gllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasiaakggvtlh
pnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtvvksgrthl
mdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapddfgvrvvav
lvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgsgpltglae
iqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnvyipmmarn
ilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyeeaaavakqa
lkerktirqtvidrgligdrlsiedldrrldvlamakaeq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4apbD (D:)
gavdadsanyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkga
caqvnsdlgllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasia
akggvtlhpnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtv
vksgrthlmdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapdd
fgvrvvavlvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgs
gpltglaeiqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnv
yipmmarnilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyee
aaavakqalkerktirqtvidrgligdrlsiedldrrldvlamakaeqldsdrl

    Sequence, based on observed residues (ATOM records): (download) 
    >4apbD (D:)
nyriehdtmgevrvpakalwraqtqravenfpisgrglertqiralgllkgacaqvnsdl
gllapekadaiiaaaaeiadgqhddqfpidvfqtgsgtssnmntneviasiaakggvtlh
pnddvnmsqssndtfptathiaateaavahlipalqqlhdalaakaldwhtvvksgrthl
mdavpvtlgqefsgyarqieagiervraclprlgelaiggtavgtglnapddfgvrvvav
lvaqtglselrtaansfeaqaardglveasgalrtiavsltkiandirwmgsgpltglae
iqlpdlqpgcsimpgkvnpvlpeavtqvaaqvigndaaiawggangafelnvyipmmarn
ilesfklltnvsrlfaqrciagltanvehlrrlaesspsivtplnsaigyeeaaavakqa
lkerktirqtvidrgligdrlsiedldrrldvlamakaeqld