PDB entry 3wyq

View 3wyq on RCSB PDB site
Description: Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution
Class: biotin binding protein
Keywords: beta-barrel, BIOTIN BINDING PROTEIN
Deposited on 2014-09-05, released 2014-12-24
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-12-24, with a file datestamp of 2014-12-19.
Experiment type: XRAY
Resolution: 1 Å
R-factor: 0.138
AEROSPACI score: 0.92 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629 (0-End)
      • engineered mutation (9)
      • engineered mutation (70-71)
      • engineered mutation (88)
      • engineered mutation (90)
      • engineered mutation (103)
    Domains in SCOPe 2.08: d3wyqa_
  • Chain 'B':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (9)
      • engineered mutation (70-71)
      • engineered mutation (88)
      • engineered mutation (90)
      • engineered mutation (103)
    Domains in SCOPe 2.08: d3wyqb_
  • Heterogens: BTN, GOL, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3wyqA (A:)
aeagitgtwsnqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
lgwtvawknnsknahsattwsgqyvggadakintqwlltsgttnanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >3wyqA (A:)
aeagitgtwsnqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
lgwtvawknnsknahsattwsgqyvggadakintqwlltsgttnanawkstlvghdtftk
vk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3wyqB (B:)
aeagitgtwsnqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgta
lgwtvawknnsknahsattwsgqyvggadakintqwlltsgttnanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >3wyqB (B:)
eagitgtwsnqlgstfivtagadgaltgtyesavgnaesryvltgrydsapatdgsgtal
gwtvawknnsknahsattwsgqyvggadakintqwlltsgttnanawkstlvghdtftkv