PDB entry 3v21

View 3v21 on RCSB PDB site
Description: Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Class: DNA binding protein, hydrolase/DNA
Keywords: RESTRICTION ENDONUCLEASE, protein-DNA complex, HYDROLASE, DNA BINDING PROTEIN, HYDROLASE-DNA complex
Deposited on 2011-12-11, released 2012-04-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-11-14, with a file datestamp of 2012-11-09.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.234
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21a_
  • Chain 'B':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21b_
  • Chain 'C':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21c_
  • Chain 'D':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21d_
  • Chain 'E':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21e_
  • Chain 'F':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21f_
  • Chain 'G':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21g_
  • Chain 'H':
    Compound: Endonuclease Bse634IR
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: bse634IR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8RT53
      • see remark 999 (109-110)
      • see remark 999 (129)
      • engineered mutation (225)
    Domains in SCOPe 2.08: d3v21h_
  • Chain 'I':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'J':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'K':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'L':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'M':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'N':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'O':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'P':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'R':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'S':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'V':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'X':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'Y':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Chain 'Z':
    Compound: DNA (5'-d(*tp*tp*cp*gp*ap*cp*cp*gp*gp*tp*cp*gp*a)-3')
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3v21A (A:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21A (A:)
nltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgl
aiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrek
lhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdie
gkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassep
vskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3v21B (B:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21B (B:)
nltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgl
aiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrek
lhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdie
gkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassep
vskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3v21C (C:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21C (C:)
ltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgla
iptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrekl
hqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdieg
kckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassepv
skadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3v21D (D:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21D (D:)
nltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgl
aiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrek
lhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdie
gkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassep
vskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3v21E (E:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21E (E:)
nltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgl
aiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrek
lhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdie
gkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassep
vskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3v21F (F:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21F (F:)
tnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakglai
ptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetreklh
qleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdiegk
ckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassepvs
kadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3v21G (G:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21G (G:)
nltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakgl
aiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetrek
lhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdie
gkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygassep
vskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >3v21H (H:)
mttnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvka
kglaiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnet
reklhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfk
diegkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygas
sepvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiikk

    Sequence, based on observed residues (ATOM records): (download) 
    >3v21H (H:)
tnltnsncveeykengktkirikpfnalielyhhqtptgsikenldklenyvkdvvkakg
laiptsgafsntrgtwfevmiaiqswnyrvkrelndyliikmpnvktfdfrkifdnetre
klhqleksllthkqqvrlitsnpdlliirqkdlikseynlpinklthenidvaltlfkdi
egkckwdslvagvglktslrpdrrlqlvhegnilkslfahlkmaywnpkaefkyygasse
pvskadddalqtaathtivnvnstperavddifsltsfedidkmldqiik

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.

  • Chain 'M':
    No sequence available.

  • Chain 'N':
    No sequence available.

  • Chain 'O':
    No sequence available.

  • Chain 'P':
    No sequence available.

  • Chain 'R':
    No sequence available.

  • Chain 'S':
    No sequence available.

  • Chain 'V':
    No sequence available.

  • Chain 'X':
    No sequence available.

  • Chain 'Y':
    No sequence available.

  • Chain 'Z':
    No sequence available.