PDB entry 3tky

View 3tky on RCSB PDB site
Description: Monolignol o-methyltransferase (momt)
Class: transferase
Keywords: directed evolution, saturation mutagenesis, regioselectivity, transferase
Deposited on 2011-08-29, released 2012-08-29
The last revision prior to the SCOPe 2.06 freeze date was dated 2012-09-05, with a file datestamp of 2012-08-31.
Experiment type: XRAY
Resolution: 2.47 Å
R-factor: 0.193
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385
      • engineered mutation (132)
      • engineered mutation (164)
      • engineered mutation (174)
    Domains in SCOPe 2.06: d3tkya1, d3tkya2
  • Chain 'B':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385
      • engineered mutation (132)
      • engineered mutation (164)
      • engineered mutation (174)
    Domains in SCOPe 2.06: d3tkyb1, d3tkyb2
  • Chain 'C':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385
      • engineered mutation (132)
      • engineered mutation (164)
      • engineered mutation (174)
    Domains in SCOPe 2.06: d3tkyc1, d3tkyc2
  • Chain 'D':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385
      • engineered mutation (132)
      • engineered mutation (164)
      • engineered mutation (174)
    Domains in SCOPe 2.06: d3tkyd1, d3tkyd2
  • Heterogens: N7I, SAH, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3tkyA (A:)
mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
nedgvslapflllatdkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfn
kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
vmeflkta

    Sequence, based on observed residues (ATOM records): (download) 
    >3tkyA (A:)
ssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeiaaqlpttnpe
apvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvslapflllat
dkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfnkgmssnstitmkkil
emyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlggdm
fdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsiatkv
vihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkt

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3tkyB (B:)
mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
nedgvslapflllatdkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfn
kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
vmeflkta

    Sequence, based on observed residues (ATOM records): (download) 
    >3tkyB (B:)
iqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvgyispaeiaaqlpt
tnpeapvmldrvlrllasysvvtytlrerlyglapvckfltknedgvslapflllatdkv
llepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfnkgmssnstitmkkilemy
ngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlggdmfdg
vpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsiatkvvih
tdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkt

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3tkyC (C:)
mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
nedgvslapflllatdkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfn
kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
vmeflkta

    Sequence, based on observed residues (ATOM records): (download) 
    >3tkyC (C:)
ssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeiaaqlpttnpe
apvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvslapflllat
dkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfnkgmssnstitmkkil
emyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlggdm
fdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsiatkv
vihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkt

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3tkyD (D:)
mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
nedgvslapflllatdkvllepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfn
kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
vmeflkta

    Sequence, based on observed residues (ATOM records): (download) 
    >3tkyD (D:)
iqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksgyispaeiaaqlptt
npeapvmldrvlrllasysvvtytlrerlyglapvckfltknedgvslapflllatdkvl
lepwfylkdaileggipfnkaygmnifdyhgtdhrinkvfnkgmssnstitmkkilemyn
gfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlggdmfdgv
pkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsiatkvviht
dalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkt