PDB entry 3stg

View 3stg on RCSB PDB site
Description: Crystal structure of A58P, DEL(N59), and loop 7 truncated mutant of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis
Class: transferase
Keywords: manno-octulosonate, synthase, lipopolysaccharide, kdop, kdo8 kdops, kdo8ps, tim barrel, biosynthesis, transferase, lyase, lipopolysaccharide biosynthesis
Deposited on 2011-07-09, released 2011-11-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-11-23, with a file datestamp of 2011-11-18.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.199
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Neisseria meningitidis [TaxId:122586]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-267)
      • engineered mutation (57)
    Domains in SCOPe 2.08: d3stga_
  • Chain 'B':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Neisseria meningitidis [TaxId:122586]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-267)
      • engineered mutation (57)
    Domains in SCOPe 2.08: d3stgb_
  • Chain 'C':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Neisseria meningitidis [TaxId:122586]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-267)
      • engineered mutation (57)
    Domains in SCOPe 2.08: d3stgc_
  • Chain 'D':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Neisseria meningitidis [TaxId:122586]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-267)
      • engineered mutation (57)
    Domains in SCOPe 2.08: d3stgd_
  • Heterogens: CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3stgA (A:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpklakcdgpsal
plhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3stgA (A:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpsalplhlledf
lirikalddliksqpiltie

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3stgB (B:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpklakcdgpsal
plhlledflirikalddliksqpiltie

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3stgC (C:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpklakcdgpsal
plhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3stgC (C:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpsalplhlledf
lirikalddliksqpiltie

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3stgD (D:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpdpklakcdgpsal
plhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3stgD (D:)
mdikinditlgnnspfvlfgginvlesldstlqtcahyvevtrklgipyifkasfdkprs
sihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtdl
vvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlgf
gvmkqtcgnlpvifdvthslgrraqaldlalagmatrlaglfleshpsalplhlledfli
rikalddliksqpiltie