PDB entry 3sqk

View 3sqk on RCSB PDB site
Description: Conversion of the enzyme guanylate kinase into a mitotic-spindle orienting protein by a single mutation that inhibits GMP-induced closing
Class: Transferase
Keywords: Phosphoryl transferase, Transferase
Deposited on 2011-07-05, released 2011-10-12
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-06-13, with a file datestamp of 2012-06-08.
Experiment type: XRAY
Resolution: 2.45 Å
R-factor: 0.221
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanylate kinase
    Species: Saccharomyces cerevisiae [TaxId:559292]
    Gene: D9461.39, Guanylate Kinase 1, GUK1, YDR454C
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15454 (14-End)
      • expression tag (10-13)
      • engineered mutation (48)
    Domains in SCOPe 2.08: d3sqka1, d3sqka2
  • Chain 'B':
    Compound: Guanylate kinase
    Species: Saccharomyces cerevisiae [TaxId:559292]
    Gene: D9461.39, Guanylate Kinase 1, GUK1, YDR454C
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15454 (14-End)
      • expression tag (0-13)
      • engineered mutation (48)
    Domains in SCOPe 2.08: d3sqkb1, d3sqkb2
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3sqkA (A:)
    didiatenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtpragev
    ngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgvk
    svkaipelnarflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdkv
    ivnddldkaykelkdfifaek
    

    Sequence, based on observed residues (ATOM records): (download)
    >3sqkA (A:)
    fqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtpragevngkdynfvsv
    defksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgvksvkaipelna
    rflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdkvivnddldkay
    kelkdfifa
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3sqkB (B:)
    didiatenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtpragev
    ngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgvk
    svkaipelnarflfiappsvedlkkrlegrgteteesinkrlsaaqaelayaetgahdkv
    ivnddldkaykelkdfifaek
    

    Sequence, based on observed residues (ATOM records): (download)
    >3sqkB (B:)
    didiatenlyfqgsmsrpivisgpsgtgkstllkklfaeypdsfgfsvpsttrtpragev
    ngkdynfvsvdefksmiknnefiewaqfsgnyygstvasvkqvsksgktcildidmqgvk
    svkaipelnarflfiappsvedlkkkrlsaaqaelayaetgahdkvivnddldkaykelk
    dfifa