PDB entry 3sev

View 3sev on RCSB PDB site
Description: Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization
Class: sugar binding protein
Keywords: metal-mediated synthetic symmetrization, synthetic symmetrization, SUGAR BINDING PROTEIN
Deposited on 2011-06-11, released 2011-09-21
The last revision prior to the SCOPe 2.06 freeze date was dated 2011-11-02, with a file datestamp of 2011-10-28.
Experiment type: XRAY
Resolution: 3.05 Å
R-factor: 0.223
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Maltose-binding periplasmic protein
    Species: Escherichia coli [TaxId:83333]
    Gene: b4034, JW3994, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (1-358)
      • engineered mutation (309)
      • engineered mutation (313)
      • expression tag (359-370)
    Domains in SCOPe 2.06: d3seva1, d3seva2
  • Chain 'C':
    Compound: Maltose-binding periplasmic protein
    Species: Escherichia coli [TaxId:83333]
    Gene: b4034, JW3994, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (1-358)
      • engineered mutation (309)
      • engineered mutation (313)
      • expression tag (359-370)
    Domains in SCOPe 2.06: d3sevc1, d3sevc2
  • Chain 'E':
    Compound: Maltose-binding periplasmic protein
    Species: Escherichia coli [TaxId:83333]
    Gene: b4034, JW3994, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (1-358)
      • engineered mutation (309)
      • engineered mutation (313)
      • expression tag (359-370)
    Domains in SCOPe 2.06: d3seve1, d3seve2
  • Heterogens: MAL, ZN, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3sevA (A:)
mkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdi
ifwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynk
dllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydik
dvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsk
vnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkpl
gavalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvda
alaaaqtnaaaa

    Sequence, based on observed residues (ATOM records): (download) 
    >3sevA (A:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdaa
laaaqtnaaa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3sevC (C:)
mkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdi
ifwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynk
dllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydik
dvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsk
vnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkpl
gavalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvda
alaaaqtnaaaa

    Sequence, based on observed residues (ATOM records): (download) 
    >3sevC (C:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdaa
laaaqtnaaa

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3sevE (E:)
mkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdi
ifwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynk
dllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydik
dvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsk
vnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkpl
gavalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvda
alaaaqtnaaaa

    Sequence, based on observed residues (ATOM records): (download) 
    >3sevE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyehelahdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdaa
laaaqtnaaa