PDB entry 3rgw

View 3rgw on RCSB PDB site
Description: Crystal structure at 1.5 A resolution of an H2-reduced, O2-tolerant hydrogenase from Ralstonia eutropha unmasks a novel iron-sulfur cluster
Class: oxidoreductase/oxidoreductase
Keywords: [NIFE] hydrogenase, high-resolution crystal structure, Knallgasbacteria, proteobacteria, aerobic hydrogen bacteria, dehydrogenase, oxidoreductase, hydrogen, dihydrogen, hydrogen catalysis, metalloenzyme, metalloprotein catalytic center, nickel, iron, nickel-iron cofactor, bimetallic, Ni-Fe active site, iron-sulfur cluster, T-cluster, [4Fe-3S] cluster, [3Fe-4S] cluster, [4Fe-4S] cluster, reduced state, oxygen-tolerant hydrogenase, membrane, membrane-bound, OXIDOREDUCTASE-OXIDOREDUCTASE complex
Deposited on 2011-04-10, released 2011-10-26
The last revision prior to the SCOPe 2.05 freeze date was dated 2011-11-23, with a file datestamp of 2011-11-18.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: 0.14
AEROSPACI score: 0.67 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'L':
    Compound: Membrane-bound hydrogenase (NIFE) large subunit HOXG
    Species: Ralstonia eutropha [TaxId:381666]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.05: d3rgwl_
  • Chain 'S':
    Compound: Membrane-bound hydrogenase (NIFE) small subunit HOXK
    Species: Ralstonia eutropha [TaxId:381666]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.05: d3rgws_
  • Heterogens: NFU, MG, SF4, F3S, F4S, HOH

PDB Chain Sequences:

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >3rgwL (L:)
msayatqgfnlddrgrrivvdpvtrieghmrcevnvdannvirnavstgtmwrglevilk
grdprdawafvericgvctgchalasvravenaldiripknahlireimaktlqvhdhav
hfyhlhaldwvdvmsalkadpkrtselqqlvspahplssagyfrdiqnrlkrfvesgqlg
pfmngywgskayvlppeanlmavthylealdlqkewvkihtifggknphpnylvggvpca
inldgigaasapvnmerlsfvkarideiiefnknvyvpdvlaigtlykqagwlyggglaa
tnvldygeypnvaynkstdqlpggailngnwdevfpvdprdsqqvqefvshswykyades
vglhpwdgvtepnyvlgantkgtrtrieqidesakyswiksprwrghamevgplsryila
yaharsgnkyaerpkeqleysaqminsaipkalglpetqytlkqllpstigrtlarales
qycgemmhsdwhdlvaniragdtatanvdkwdpatwplqakgvgtvaaprgalghwirik
dgrienyqcvvpttwngsprdykgqigafeaslmntpmvnpeqpveilrtlhsfdpclac
sth

    Sequence, based on observed residues (ATOM records): (download) 
    >3rgwL (L:)
sayatqgfnlddrgrrivvdpvtrieghmrcevnvdannvirnavstgtmwrglevilkg
rdprdawafvericgvctgchalasvravenaldiripknahlireimaktlqvhdhavh
fyhlhaldwvdvmsalkadpkrtselqqlvspahplssagyfrdiqnrlkrfvesgqlgp
fmngywgskayvlppeanlmavthylealdlqkewvkihtifggknphpnylvggvpcai
nldgigaasapvnmerlsfvkarideiiefnknvyvpdvlaigtlykqagwlyggglaat
nvldygeypnvaynkstdqlpggailngnwdevfpvdprdsqqvqefvshswykyadesv
glhpwdgvtepnyvlgantkgtrtrieqidesakyswiksprwrghamevgplsryilay
aharsgnkyaerpkeqleysaqminsaipkalglpetqytlkqllpstigrtlaralesq
ycgemmhsdwhdlvaniragdtatanvdkwdpatwplqakgvgtvaaprgalghwirikd
grienyqcvvpttwngsprdykgqigafeaslmntpmvnpeqpveilrtlhsfdpclacs
th

  • Chain 'S':
    Sequence, based on SEQRES records: (download) 
    >3rgwS (S:)
metkprtpvlwlhglectccsesfirsahplakdvvlsmisldyddtlmaaaghqaeail
eeimtkykgnyilavegnpplnqdgmsciiggrpfieqlkyvakdakaiiswgscaswgc
vqaakpnptqatpvhkvitdkpiikvpgcppiaevmtgvitymltfdripeldrqgrpkm
fysqrihdkcyrrphfdagqfveewddesarkgfclykmgckgpttynacsttrwnegts
fpiqsghgcigcsedgfwdkgsfydrltgisqfgveanadkiggtasvvvgaavtahaaa
saikraskknetsgsehrsawshpqfekrsawshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >3rgwS (S:)
prtpvlwlhglectccsesfirsahplakdvvlsmisldyddtlmaaaghqaeaileeim
tkykgnyilavegnpplnqdgmsciiggrpfieqlkyvakdakaiiswgscaswgcvqaa
kpnptqatpvhkvitdkpiikvpgcppiaevmtgvitymltfdripeldrqgrpkmfysq
rihdkcyrrphfdagqfveewddesarkgfclykmgckgpttynacsttrwnegtsfpiq
sghgcigcsedgfwdkgsfydrltgisqfg