PDB entry 3rdo

View 3rdo on RCSB PDB site
Description: Crystal structure of R7-2 streptavidin complexed with biotin
Class: biotin binding protein
Keywords: Streptavidin variants, improved desthiobiotin binding, opened loop destabilization, BIOTIN BINDING PROTEIN
Deposited on 2011-04-01, released 2011-07-06
The last revision prior to the SCOPe 2.07 freeze date was dated 2011-07-06, with a file datestamp of 2011-07-01.
Experiment type: XRAY
Resolution: 1.4 Å
R-factor: 0.152
AEROSPACI score: 0.7 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629 (25-End)
      • expression tag (6-10)
      • expression tag (24)
      • engineered mutation (41)
      • engineered mutation (64-65)
      • engineered mutation (102)
      • engineered mutation (120)
      • engineered mutation (122)
    Domains in SCOPe 2.07: d3rdoa1, d3rdoa2
  • Heterogens: BTN, NI, SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3rdoA (A:)
    msgshhhhhhssgiegrgrlikhmtaeagitgtwynqlgstlivtagadgaltgtyesav
    gnaegsyvltgrydsapatdgsgtalgwtvawknnyrnahsastwsgqyvggaearintq
    vlttsgtteanawkstlvghdtftkvkpsaasi
    

    Sequence, based on observed residues (ATOM records): (download)
    >3rdoA (A:)
    hhhhstaeagitgtwynqlgstlivtagadgaltgtyesavgnaegsyvltgrydsapat
    dgsgtalgwtvawknnyrnahsastwsgqyvggaearintqvlttsgtteanawkstlvg
    hdtftkvkp