PDB entry 3qzu

View 3qzu on RCSB PDB site
Description: Crystal structure of Bacillus subtilis Lipase A 7-fold mutant; the outcome of directed evolution towards thermostability
Class: hydrolase
Keywords: Alpha/beta hydrolase, HYDROLASE
Deposited on 2011-03-07, released 2012-02-08
The last revision prior to the SCOPe 2.07 freeze date was dated 2012-04-18, with a file datestamp of 2012-04-13.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: 0.175
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lipase estA
    Species: Bacillus subtilis subsp. subtilis [TaxId:224308]
    Gene: BSU02700, estA, lip, lipA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37957 (Start-180)
      • engineered mutation (32-34)
      • engineered mutation (111)
      • engineered mutation (133)
      • engineered mutation (138)
      • engineered mutation (156)
    Domains in SCOPe 2.07: d3qzua_
  • Chain 'B':
    Compound: Lipase estA
    Species: Bacillus subtilis subsp. subtilis [TaxId:224308]
    Gene: BSU02700, estA, lip, lipA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37957 (Start-180)
      • engineered mutation (32-34)
      • engineered mutation (111)
      • engineered mutation (133)
      • engineered mutation (138)
      • engineered mutation (156)
    Domains in SCOPe 2.07: d3qzub_
  • Heterogens: CL, SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3qzuA (A:)
aehnpvvmvhgiggasfnfagiksylvsqgwsqndlyavdfwdktgtnynngpvlsrfvq
kvldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgdalpgtdpn
qkilytsiyssaddivmnclsrldgarnvqihgvghmgllyssqvnslikeglngggqnt
n

    Sequence, based on observed residues (ATOM records): (download) 
    >3qzuA (A:)
ehnpvvmvhgiggasfnfagiksylvsqgwsqndlyavdfwdktgtnynngpvlsrfvqk
vldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgdalpgtdpnq
kilytsiyssaddivmnclsrldgarnvqihgvghmgllyssqvnslikeglngggqntn

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3qzuB (B:)
aehnpvvmvhgiggasfnfagiksylvsqgwsqndlyavdfwdktgtnynngpvlsrfvq
kvldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgdalpgtdpn
qkilytsiyssaddivmnclsrldgarnvqihgvghmgllyssqvnslikeglngggqnt
n

    Sequence, based on observed residues (ATOM records): (download) 
    >3qzuB (B:)
ehnpvvmvhgiggasfnfagiksylvsqgwsqndlyavdfwdktgtnynngpvlsrfvqk
vldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgdalpgtdpnq
kilytsiyssaddivmnclsrldgarnvqihgvghmgllyssqvnslikeglngggqntn