PDB entry 3puy

View 3puy on RCSB PDB site
Description: Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to AMP-PNP after crystal soaking of the pretranslocation state
Class: transport protein, membrane protein
Keywords: ATP Binding Cassette, Nucleotide Binding Domain, Substrate Binding Protein, ABC Transporter, importer, ATPase, ATP binding, Maltodextrin binding, transmembrane integral membrane, TRANSPORT PROTEIN, MEMBRANE PROTEIN
Deposited on 2010-12-06, released 2011-05-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 3.1 Å
R-factor: N/A
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Fused maltose transport subunit, ATP-binding component of ABC superfamily; regulatory protein
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4224, malK
    Database cross-references and differences (RAF-indexed):
    • Uniprot B1XC34 (Start-370)
      • expression tag (371)
    Domains in SCOPe 2.08: d3puya1, d3puya2, d3puya3
  • Chain 'B':
    Compound: Fused maltose transport subunit, ATP-binding component of ABC superfamily; regulatory protein
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4224, malK
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3puyb1, d3puyb2
  • Chain 'E':
    Compound: Maltose transporter subunit; periplasmic-binding component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4223, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot B1XC33 (0-369)
      • expression tag (370-373)
    Domains in SCOPe 2.08: d3puye1, d3puye2
  • Chain 'F':
    Compound: Maltose transporter subunit; membrane component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4222, malF
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3puyf1, d3puyf2
  • Chain 'G':
    Compound: Maltose transporter subunit; membrane component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4221, malG
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3puyg_
  • Heterogens: PGV, MG, ANP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3puyA (A:)
masvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdl
figekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaev
lqlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlh
krlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmn
flpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvil
egevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgt
acrrlhkepgvasashhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puyA (A:)
asvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdlf
igekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaevl
qlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlhk
rlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmnf
lpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvile
gevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgta
crrlhkepgva

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3puyB (B:)
masvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdl
figekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaev
lqlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlh
krlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmn
flpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvil
egevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgt
acrrlhkepgvasashhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puyB (B:)
asvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdlf
igekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaevl
qlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlhk
rlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmnf
lpvkataidqvqvelpmpnrqqvwlpvenmslgirpehllpsdiadvilegevqvveqlg
netqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgtacrrlhkep

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3puyE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdea
lkdaqtritkasashhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puyE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdea
lkdaqtritkasas

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3puyF (F:)
mdvikkkhwwqsdalkwsvlgllgllvgylvvlmyaqgeylfaittlilssaglyifanr
kayawryvypgmagmglfvlfplvctiaiaftnysstnqltferaqevlldrswqagkty
nfglypagdewqlalsdgetgknylsdafkfggeqklqlkettaqpegeranlrvitqnr
qalsditailpdgnkvmmsslrqfsgtqplytldgdgtltnnqsgvkyrpnnqigfyqsi
tadgnwgdeklspgytvttgwknftrvftdegiqkpflaifvwtvvfslitvfltvavgm
vlaclvqwealrgkavyrvllilpyavpsfisilifkglfnqsfgeinmmlsalfgvkpa
wfsdpttartmliivntwlgypymmilcmgllkaipddlyeasamdgagpfqnffkitlp
llikpltplmiasfafnfnnfvliqlltnggpdrlgtttpagytdllvnytyriafeggg
gqdfglaaaiatlifllvgalaivnlkatrmkfd

    Sequence, based on observed residues (ATOM records): (download) 
    >3puyF (F:)
wqsdalkwsvlgllgllvgylvvlmyaqgeylfaittlilssaglyifanrkayawryvy
pgmagmglfvlfplvctiaiaftnysstnqltferaqevlldrswqagktynfglypagd
ewqlalsdgetgknylsdafkfggeqklqlkettaqpegeranlrvitqnrqalsditai
lpdgnkvmmsslrqfsgtqplytldgdgtltnnqsgvkyrpnnqigfyqsinwgdeklsp
gytvttgwknftrvftdegiqkpflaifvwtvvfslitvfltvavgmvlaclvqwealrg
kavyrvllilpyavpsfisilifkglfnqsfgeinmmlsalfgvkpawfsdpttartmli
ivntwlgypymmilcmgllkaipddlyeasamdgagpfqnffkitlpllikpltplmias
fafnfnnfvliqlltnggpdrlgtttpagytdllvnytyriafeggggqdfglaaaiatl
ifllvgalai

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3puyG (G:)
mamvqpksqkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwk
lalgfsveqadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkat
llkgmlifqmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgy
fetidssleeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdv
nsytlavgmqqylnpqnylwgdfaaaavmsalpitivfllaqrwlvngltaggvkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3puyG (G:)
amvqpqkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwklal
gfsveqadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkatllk
gmlifqmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgyfet
idssleeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdvnsy
tlavgmqqylnpqnylwgdfaaaavmsalpitivfllaqrwlvngltaggvkg