PDB entry 3ows

View 3ows on RCSB PDB site
Description: Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M116C-CN from P. putida with Bound Equilenin
Class: isomerase
Keywords: Isomerase, Steroid, Cyanylation
Deposited on 2010-09-20, released 2011-09-21
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-07-17, with a file datestamp of 2013-07-12.
Experiment type: XRAY
Resolution: 1.71 Å
R-factor: 0.215
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (39)
      • engineered mutation (68)
      • engineered mutation (80)
      • engineered mutation (96)
      • engineered mutation (115)
    Domains in SCOPe 2.08: d3owsa_
  • Chain 'B':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (39)
      • engineered mutation (68)
      • engineered mutation (80)
      • engineered mutation (96)
      • engineered mutation (115)
    Domains in SCOPe 2.08: d3owsb_
  • Chain 'C':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (39)
      • engineered mutation (68)
      • engineered mutation (80)
      • engineered mutation (96)
      • engineered mutation (115)
    Domains in SCOPe 2.08: d3owsc_
  • Chain 'D':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (39)
      • engineered mutation (68)
      • engineered mutation (80)
      • engineered mutation (96)
      • engineered mutation (115)
    Domains in SCOPe 2.08: d3owsd_
  • Heterogens: EQU, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3owsA (A:)
mnlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqg
lgggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >3owsA (A:)
nlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqgl
gggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayws
evnlsvrep

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3owsB (B:)
mnlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqg
lgggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >3owsB (B:)
nlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqgl
gggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayws
evnlsvrep

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3owsC (C:)
mnlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqg
lgggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >3owsC (C:)
nlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqgl
gggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayws
evnlsvrep

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3owsD (D:)
mnlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqg
lgggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >3owsD (D:)
nlptaqevqglmaryielvdvgdieaivqmyaddatvenpfgqppihgreqiaafyrqgl
gggkvrasltgpvrashngsgampfrvemvwngqpsaldvidvmrfdehgriqtxqayws
evnlsvrep