PDB entry 3otu

View 3otu on RCSB PDB site
Description: PDK1 mutant bound to allosteric disulfide fragment activator JS30
Class: transferase/transferase activator
Keywords: PIF pocket, activation loop, C helix, Ser/Thr-kinase, AGC kinase, allostery, transferase, allosteric activator, bisindolylmaleimide, phosphorylation, disulfide, kinase, PDK1, TRANSFERASE-TRANSFERASE ACTIVATOR complex
Deposited on 2010-09-13, released 2011-03-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-04-27, with a file datestamp of 2011-04-22.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.197
AEROSPACI score: 0.4 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 3-phosphoinositide-dependent protein kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: PDK1, PDPK1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O15530 (Start-315)
      • engineered mutation (104)
    Domains in SCOPe 2.08: d3otua_
  • Heterogens: J30, BI4, SO4, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3otuA (A:)
gamdpefmdgtaaeprpgagslqhaqpppqprkkrpedfkfgkilgegsfstvvlarela
tsreyaikilekrhiikenkvpyvtrerdvmsrldhpffvklyfcfqddeklyfglsyak
ngellkyirkigsfdetctrfytaeivsaleylhgkgiihrdlkpenillnedmhiqitd
fgtakvlspeskqaransfvgtaqyvspellteksackssdlwalgciiyqlvaglppfr
agneylifqkiikleydfpekffpkardlvekllvldatkrlgceemegygplkahpffe
svtwenlhqqtppklt

    Sequence, based on observed residues (ATOM records): (download) 
    >3otuA (A:)
krpedfkfgkilgesfstvvlarelatsreyaikilekrhiikenkvpyvtrerdvmsrl
dhpffvklyfcfqddeklyfglsyakngellkyirkigsfdetctrfytaeivsaleylh
gkgiihrdlkpenillnedmhiqitdfgtakvlsaransfvgtaqyvspellteksacks
sdlwalgciiyqlvaglppfragneylifqkiikleydfpekffpkardlvekllvldat
krlgceemegygplkahpffesvtwenlhqqtppklt