PDB entry 3o69

View 3o69 on RCSB PDB site
Description: Structure of the E100A E.coli GDP-mannose hydrolase (yffh) in complex with Mg++
Class: hydrolase
Keywords: nudix, GDP_mannose, hydrolase, biofilm
Deposited on 2010-07-28, released 2011-05-11
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-08-10, with a file datestamp of 2011-08-05.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.208
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: GDP-mannose pyrophosphatase nudK
    Species: Escherichia coli [TaxId:83333]
    Gene: b2467, JW2451, nudK, yffH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37128 (Start-190)
      • engineered mutation (99)
    Domains in SCOPe 2.08: d3o69a_
  • Chain 'B':
    Compound: GDP-mannose pyrophosphatase nudK
    Species: Escherichia coli [TaxId:83333]
    Gene: b2467, JW2451, nudK, yffH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37128 (Start-190)
      • engineered mutation (99)
    Domains in SCOPe 2.08: d3o69b_
  • Heterogens: MG, PEG, NA, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3o69A (A:)
mtqqitlikdkilsdnyftlhnitydltrkdgevirhkrevydrgngatillyntkkktv
vlirqfrvatwvngnesgqliescaglldndepevcirkaaieetgyevgevrklfelym
spggvtelihffiaeysdnqranagggvededievlelpfsqalemiktgeirdgktvll
lnylqtshlmd

    Sequence, based on observed residues (ATOM records): (download) 
    >3o69A (A:)
tqqitlikdkilsdnyftlhnitydltrkdevirhkrevydrgngatillyntkkktvvl
irqfrvatwvngnesgqliescagllndepevcirkaaieetgyevgevrklfelymspg
gvtelihffiaeysdedievlelpfsqalemiktgeirdgktvlllnylqtshlmd

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3o69B (B:)
mtqqitlikdkilsdnyftlhnitydltrkdgevirhkrevydrgngatillyntkkktv
vlirqfrvatwvngnesgqliescaglldndepevcirkaaieetgyevgevrklfelym
spggvtelihffiaeysdnqranagggvededievlelpfsqalemiktgeirdgktvll
lnylqtshlmd

    Sequence, based on observed residues (ATOM records): (download) 
    >3o69B (B:)
qqitlikdkilsdnyftlhnitydltrkdgevirhkrevydrgngatillyntkkktvvl
irqfrvatwvngnesgqliescaglldndepevcirkaaieetgyevgevrklfelymsp
ggvtelihffiaeysdnqranagggvededievlelpfsqalemiktgeirdgktvllln
ylqtshlmd