PDB entry 3nic

View 3nic on RCSB PDB site
Description: DNA binding and cleavage by the GIY-YIG endonuclease R.Eco29kI inactive variant Y49F
Deposited on 2010-06-15, released 2010-09-08
The last revision was dated 2010-10-27, with a file datestamp of 2010-10-22.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.21
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'B':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'C':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'D':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'E':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • expression tag (21)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'F':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'G':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'H':
    Compound: Eco29kIR
    Species: Escherichia coli [TaxId:562]
    Gene: eco29kIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46944 (22-End)
      • engineered mutation (69)
      • engineered mutation (89)
  • Chain 'I':
    Compound: DNA (5'-d(p*cp*gp*gp*gp*ap*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*gp*cp*cp*gp*c)-3')
  • Chain 'J':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*cp*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*tp*cp*cp*cp*g)-3')
  • Chain 'K':
    Compound: DNA (5'-d(p*cp*gp*gp*gp*ap*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*gp*cp*cp*gp*c)-3')
  • Chain 'L':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*cp*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*tp*cp*cp*cp*g)-3')
  • Chain 'M':
    Compound: DNA (5'-d(p*cp*gp*gp*gp*ap*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*gp*cp*cp*gp*c)-3')
  • Chain 'N':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*cp*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*tp*cp*cp*cp*g)-3')
  • Chain 'O':
    Compound: DNA (5'-d(p*cp*gp*gp*gp*ap*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*gp*cp*cp*gp*c)-3')
  • Chain 'P':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*cp*gp*gp*cp*cp*cp*gp*cp*gp*gp*gp*cp*cp*tp*cp*cp*cp*g)-3')
  • Heterogens: PO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.07, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3nicA (A:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicA (A:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsks
    

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3nicB (B:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicB (B:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsks
    

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3nicC (C:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicC (C:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsk
    

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3nicD (D:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicD (D:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsk
    

  • Chain 'E':
    Sequence, based on SEQRES records:
    >3nicE (E:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicE (E:)
    ghnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghysly
    deysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldl
    cdfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvi
    hpgrewaekctgvhsepyfieerikqyfs
    

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3nicF (F:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicF (F:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsk
    

  • Chain 'G':
    Sequence, based on SEQRES records:
    >3nicG (G:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicG (G:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsk
    

  • Chain 'H':
    Sequence, based on SEQRES records:
    >3nicH (H:)
    masshhhhhhssglvprgssmghnkkfdrsehvyrndsflelikdavrffsgtpvhslpp
    perfqgagvfalyytghyslydeysrinrkaynlpiyvgkavpagwrqsrisdhetrags
    elsnrirehgrniaktsnldlcdfscrfvifeatgsdmistveaalikiykplwntvvdg
    fgnhtpgagrfaqaksdwdvihpgrewaekctgvhsepyfieerikqyfsksnft
    

    Sequence, based on observed residues (ATOM records):
    >3nicH (H:)
    hnkkfdrsehvyrndsflelikdavrffsgtpvhslppperfqgagvfalyytghyslyd
    eysrinrkaynlpiyvgkavpagwrqsrisdhetragselsnrirehgrniaktsnldlc
    dfscrfvifeatgsdmistveaalikiykplwntvvdgfgnhtpgagrfaqaksdwdvih
    pgrewaekctgvhsepyfieerikqyfsk
    

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.

  • Chain 'M':
    No sequence available.

  • Chain 'N':
    No sequence available.

  • Chain 'O':
    No sequence available.

  • Chain 'P':
    No sequence available.