PDB entry 3ngz

View 3ngz on RCSB PDB site
Description: Crystal structure of RNase T in complex with a non-preferred ssDNA (GC) with one Mg in the active site
Class: hydrolase/DNA
Keywords: exoribonuclease, RNA processing, RNA maturation, protein-DNA interactions, protein-DNA complex, exo-nuclease, HYDROLASE-DNA complex
Deposited on 2010-06-14, released 2011-02-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2011-12-21, with a file datestamp of 2011-12-16.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.2
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:562]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • engineered mutation (111)
    Domains in SCOPe 2.07: d3ngza_
  • Chain 'B':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:562]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • engineered mutation (111)
    Domains in SCOPe 2.07: d3ngzb_
  • Chain 'C':
    Compound: 5'-d(p*gp*c)-3'
  • Chain 'D':
    Compound: 5'-d(p*gp*c)-3'
  • Heterogens: MG, CO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3ngzA (A:)
    mgsshhhhhhssglvprgshmsdnaqltglcdrfrgfypvvidvetagfnaktdalleia
    aitlkmdeqgwlmpdttlhfhvepfvganlqpealafngidpndpdrgavsgyealheif
    kvvrkgikasgcnraimvahnanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalg
    qtvlskacqtagmdfdstqahsalydtertavlfceivnrwkrlggwplsaaeev
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ngzA (A:)
    tglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhfhvepfvg
    anlqpealafngidpndpdrgavsgyealheifkvvrkgikasgcnraimvahnanfdhs
    fmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqahsalydt
    ertavlfceivnrwkrlggwplsaaee
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3ngzB (B:)
    mgsshhhhhhssglvprgshmsdnaqltglcdrfrgfypvvidvetagfnaktdalleia
    aitlkmdeqgwlmpdttlhfhvepfvganlqpealafngidpndpdrgavsgyealheif
    kvvrkgikasgcnraimvahnanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalg
    qtvlskacqtagmdfdstqahsalydtertavlfceivnrwkrlggwplsaaeev
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ngzB (B:)
    tglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhfhvepfvg
    anlqpealafngidpndpdrgavsgyealheifkvvrkgikasgcnraimvahnanfdhs
    fmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqahsalydt
    ertavlfceivnrwkrlggwplsaae
    

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.