PDB entry 3nbj

View 3nbj on RCSB PDB site
Description: Crystal Structure of Y305F mutant of the copper amine oxidase from Hansenula polymorpha expressed in yeast
Deposited on 2010-06-03, released 2010-08-25
The last revision was dated 2010-09-08, with a file datestamp of 2010-09-03.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: 0.213
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Chain 'B':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Chain 'C':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Chain 'D':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Chain 'E':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Chain 'F':
    Compound: Peroxisomal primary amine oxidase
    Species: Pichia angusta [TaxId:4905]
    Gene: AMO
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12807
      • engineered mutation (304)
  • Heterogens: CU, PO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.07, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3nbjA (A:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjA (A:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav
    

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3nbjB (B:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjB (B:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav
    

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3nbjC (C:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjC (C:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav
    

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3nbjD (D:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjD (D:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav
    

  • Chain 'E':
    Sequence, based on SEQRES records:
    >3nbjE (E:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjE (E:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav
    

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3nbjF (F:)
    merlrqiasqataasaaparpahpldplstaeikaatntvksyfagkkisfntvtlrepa
    rkayiqwkeqggplpprlayyvileagkpgvkeglvdlaslsvietraletvqpiltved
    lcsteevirndpavieqcvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsd
    eddsqyshpldfcpivdteekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeap
    pinvtqpegvsfkmtgnvmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislse
    mivpfgspefphqrkhaldigeygagymtnplslgcdckgvihyldahfsdragdpitvk
    navciheeddgllfkhsdfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgair
    ldirltgilntyilgddeeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacda
    ksspyplgspenmygnafysekttfktvkdsltnyesatgrswdifnpnkvnpysgkpps
    yklvstqcppllakegslvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmre
    wigdgsenidntdilffhtfgithfpapedfplxpaepitlmlrprhfftenpgldiqps
    yamttseakravhketkdktsrlafegsccgk
    

    Sequence, based on observed residues (ATOM records):
    >3nbjF (F:)
    parpahpldplstaeikaatntvksyfagkkisfntvtlreparkayiqwkeqggplppr
    layyvileagkpgvkeglvdlaslsvietraletvqpiltvedlcsteevirndpavieq
    cvlsgipanemhkvycdpwtigyderwgtgkrlqqalvyyrsdeddsqyshpldfcpivd
    teekkvifidipnrrrkvskhkhanfypkhmiekvgamrpeappinvtqpegvsfkmtgn
    vmewsnfkfhigfnyregivlsdvsyndhgnvrpifhrislsemivpfgspefphqrkha
    ldigeygagymtnplslgcdckgvihyldahfsdragdpitvknavciheeddgllfkhs
    dfrdnfatslvtratklvvsqiftaanxeyclywvfmqdgairldirltgilntyilgdd
    eeagpwgtrvypnvnahnhqhlfslridpridgdgnsaaacdaksspyplgspenmygna
    fysekttfktvkdsltnyesatgrswdifnpnkvnpysgkppsyklvstqcppllakegs
    lvakrapwashsvnvvpykdnrlypsgdhvpqwsgdgvrgmrewigdgsenidntdilff
    htfgithfpapedfplxpaepitlmlrprhfftenpgldiqpsyamttseakrav