PDB entry 3n0f

View 3n0f on RCSB PDB site
Description: Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)
Class: lyase
Keywords: terpene cyclase fold, hemiterpene synthase, ddxxd motif, nse/dte motif, lyase
Deposited on 2010-05-13, released 2010-07-21
The last revision prior to the SCOPe 2.07 freeze date was dated 2010-10-06, with a file datestamp of 2010-10-01.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.198
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Isoprene synthase
    Species: Populus tremula x Populus alba [TaxId:80863]
    Gene: ispS
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AR86 (Start-554)
      • engineered mutation (18)
      • engineered mutation (267)
      • engineered mutation (492)
    Domains in SCOPe 2.07: d3n0fa1, d3n0fa2
  • Chain 'B':
    Compound: Isoprene synthase
    Species: Populus tremula x Populus alba [TaxId:80863]
    Gene: ispS
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AR86 (Start-554)
      • engineered mutation (18)
      • engineered mutation (267)
      • engineered mutation (492)
    Domains in SCOPe 2.07: d3n0fb1, d3n0fb2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3n0fA (A:)
    mrgshhhhhhgsearrsadyepnswdydfllssdtdesievykdkakkleaevrreinne
    kaefltllelidnvqrlglgyrfesdirraldrfvssggfdgvtktslhatalsfrllrq
    hgfevsqeafsgfkdqngnflenlkedtkailslyeasflalegenildearvfaishlk
    elseekigkelaeqvnhalelplhrrtqrleavwsieayrkkedanqvllelaildynmi
    qsvyqrdlretsrwwrrvglatklhfardrliesfywavgvafepqysdcrnsvakmfsf
    vtiiddiydvygtldelelftdaverwdvnaindlpdymklcflalyntineiaydnlkd
    kgenilpyltkawadlcnaflqeakwlynkstptfddyfgnawksssgplqlifayfavv
    qnikkeeienlqkyhdiisrpshifrlcndlasasaeiargetansvscymrtkgiseel
    atesvmnlidetwkkmnkeklggslfakpfvetainlarqshctyhngdahtspdeltrk
    rvlsvitepilpfer
    

    Sequence, based on observed residues (ATOM records): (download)
    >3n0fA (A:)
    sadyepnswdydfllssievykdkakkleaevrreinnekaefltllelidnvqrlglgy
    rfesdirraldrfvssggfdgvtktslhatalsfrllrqhgfevsqeafsgfkdqngnfl
    enlkedtkailslyeasflalegenildearvfaishlkelseekigkelaeqvnhalel
    plhrrtqrleavwsieayrkkedanqvllelaildynmiqsvyqrdlretsrwwrrvgla
    tklhfardrliesfywavgvafepqysdcrnsvakmfsfvtiiddiydvygtldelelft
    daverwdvnaindlpdymklcflalyntineiaydnlkdkgenilpyltkawadlcnafl
    qeakwlynkstptfddyfgnawksssgplqlifayfavvqnikkeeienlqkyhdiisrp
    shifrlcndlasasaeiargetansvscymrtkgiseelatesvmnlidetwkkmnkekl
    ggslfakpfvetainlarqshctyhngtspdeltrkrvlsvitepilpfer
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3n0fB (B:)
    mrgshhhhhhgsearrsadyepnswdydfllssdtdesievykdkakkleaevrreinne
    kaefltllelidnvqrlglgyrfesdirraldrfvssggfdgvtktslhatalsfrllrq
    hgfevsqeafsgfkdqngnflenlkedtkailslyeasflalegenildearvfaishlk
    elseekigkelaeqvnhalelplhrrtqrleavwsieayrkkedanqvllelaildynmi
    qsvyqrdlretsrwwrrvglatklhfardrliesfywavgvafepqysdcrnsvakmfsf
    vtiiddiydvygtldelelftdaverwdvnaindlpdymklcflalyntineiaydnlkd
    kgenilpyltkawadlcnaflqeakwlynkstptfddyfgnawksssgplqlifayfavv
    qnikkeeienlqkyhdiisrpshifrlcndlasasaeiargetansvscymrtkgiseel
    atesvmnlidetwkkmnkeklggslfakpfvetainlarqshctyhngdahtspdeltrk
    rvlsvitepilpfer
    

    Sequence, based on observed residues (ATOM records): (download)
    >3n0fB (B:)
    sadyepnswdydfllssievykdkakkleaevrreinnekaefltllelidnvqrlglgy
    rfesdirraldrfvssggfdgvtktslhatalsfrllrqhgfevsqeafsgfkdqngnfl
    enlkedtkailslyeasflalegenildearvfaishlkelseekigkelaeqvnhalel
    plhrrtqrleavwsieayrkkedanqvllelaildynmiqsvyqrdlretsrwwrrvgla
    tklhfardrliesfywavgvafepqysdcrnsvakmfsfvtiiddiydvygtldelelft
    daverwdvnaindlpdymklcflalyntineiaydnlkdkgenilpyltkawadlcnafl
    qeakwlynkstptfddyfgnawksssgplqlifayfavvqnikkeeienlqkyhdiisrp
    shifrlcndlasasaeiargetansvscymrtkgiseelatesvmnlidetwkkmnkekl
    ggslfakpfvetainlarqshctyhngtspdeltrkrvlsvitepilpfer