PDB entry 3miq

View 3miq on RCSB PDB site
Description: Structure of the GFP:GFP-nanobody complex at 2.8 A resolution in P22121
Class: luminescent protein/immune system
Keywords: GFP, GFP-nanobody, beta-barrel, antibody, LUMINESCENT PROTEIN-IMMUNE SYSTEM complex
Deposited on 2010-04-11, released 2010-06-09
The last revision prior to the SCOPe 2.07 freeze date was dated 2010-08-25, with a file datestamp of 2010-08-20.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.215
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (12-End)
      • engineered (74)
      • chromophore (75)
      • engineered (241)
    Domains in SCOPe 2.07: d3miqa_
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212
      • engineered (74)
      • chromophore (75)
    Domains in SCOPe 2.07: d3miqb_
  • Chain 'C':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (12-End)
      • engineered (74)
      • chromophore (75)
      • engineered (241)
    Domains in SCOPe 2.07: d3miqc_
  • Chain 'D':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212
      • engineered (74)
      • chromophore (75)
    Domains in SCOPe 2.07: d3miqd_
  • Chain 'E':
    Compound: GFP-nanobody
    Species: Camelus dromedarius [TaxId:9838]
    Database cross-references and differences (RAF-indexed):
    • PDB 3MIQ
    Domains in SCOPe 2.07: d3miqe_
  • Chain 'F':
    Compound: GFP-nanobody
    Species: Camelus dromedarius [TaxId:9838]
    Database cross-references and differences (RAF-indexed):
    • PDB 3MIQ
    Domains in SCOPe 2.07: d3miqf_
  • Chain 'G':
    Compound: GFP-nanobody
    Species: Camelus dromedarius [TaxId:9838]
    Database cross-references and differences (RAF-indexed):
    • PDB 3MIQ (0-End)
    Domains in SCOPe 2.07: d3miqg_
  • Chain 'H':
    Compound: GFP-nanobody
    Species: Camelus dromedarius [TaxId:9838]
    Database cross-references and differences (RAF-indexed):
    • PDB 3MIQ
    Domains in SCOPe 2.07: d3miqh1, d3miqh2
  • Heterogens: IPA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3miqA (A:)
    mahhhhhhssgvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkfict
    tgklpvpwptlvttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktr
    aevkfegdtlvnrielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirh
    niedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagi
    tlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqA (A:)
    skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
    ttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvn
    rielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladh
    yqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagitlgmde
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3miqB (B:)
    mahhhhhhssgvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkfict
    tgklpvpwptlvttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktr
    aevkfegdtlvnrielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirh
    niedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagi
    tlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqB (B:)
    kgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlvt
    tlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnr
    ielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhy
    qqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagi
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3miqC (C:)
    mahhhhhhssgvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkfict
    tgklpvpwptlvttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktr
    aevkfegdtlvnrielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirh
    niedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagi
    tlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqC (C:)
    skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
    ttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvn
    rielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladh
    yqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagitl
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >3miqD (D:)
    mahhhhhhssgvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkfict
    tgklpvpwptlvttlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktr
    aevkfegdtlvnrielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirh
    niedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagi
    tlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqD (D:)
    kgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlvt
    tlgygvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnr
    ielkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhy
    qqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >3miqE (E:)
    mqvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrss
    yedsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvsskhhh
    hhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqE (E:)
    qvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrssy
    edsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvss
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >3miqF (F:)
    mqvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrss
    yedsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvsskhhh
    hhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqF (F:)
    qvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrssy
    edsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvss
    

  • Chain 'G':
    Sequence, based on SEQRES records: (download)
    >3miqG (G:)
    mqvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrss
    yedsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvsskhhh
    hhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqG (G:)
    mqvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrss
    yedsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvss
    

  • Chain 'H':
    Sequence, based on SEQRES records: (download)
    >3miqH (H:)
    mqvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrss
    yedsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvsskhhh
    hhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >3miqH (H:)
    qvqlvesggalvqpggslrlscaasgfpvnrysmrwyrqapgkerewvagmssagdrssy
    edsvkgrftisrddarntvylqmnslkpedtavyycnvnvgfeywgqgtqvtvssk