PDB entry 3iwu

View 3iwu on RCSB PDB site
Description: Crystal structure of Y116T/I16A double mutant of 5-hydroxyisourate hydrolase
Class: hydrolase
Keywords: HIUase, Transthyretin, TRP, thyroid hormones, molecular evolution, HYDROLASE, Peroxisome, Purine metabolism
Deposited on 2009-09-03, released 2010-09-01
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-01-01, with a file datestamp of 2019-12-27.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: N/A
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwua_
  • Chain 'B':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwub_
  • Chain 'C':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwuc_
  • Chain 'D':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwud_
  • Chain 'E':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwue_
  • Chain 'F':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwuf_
  • Chain 'G':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwug_
  • Chain 'H':
    Compound: 5-hydroxyisourate hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: urah
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06S87 (Start-137)
      • engineered mutation (34)
      • engineered mutation (134)
    Domains in SCOPe 2.08: d3iwuh_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3iwuA (A:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuA (A:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3iwuB (B:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuB (B:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3iwuC (C:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuC (C:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >3iwuD (D:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuD (D:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >3iwuE (E:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuE (E:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >3iwuF (F:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuF (F:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'G':
    Sequence, based on SEQRES records: (download)
    >3iwuG (G:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuG (G:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs
    

  • Chain 'H':
    Sequence, based on SEQRES records: (download)
    >3iwuH (H:)
    mnrlqhirghivsadkhinmsatllsplsthvlnaaqgvpganmtivlhrldpvssawni
    lttgitnddgrcpglitkenfiagvykmrfetgkywdalgetcfypyveivftitntsqh
    yhvplllsrfsysttrgs
    

    Sequence, based on observed residues (ATOM records): (download)
    >3iwuH (H:)
    lsplsthvlnaaqgvpganmtivlhrldpvssawnilttgitnddgrcpglitkenfiag
    vykmrfetgkywdalgetcfypyveivftitntsqhyhvplllsrfsysttrgs