PDB entry 3i3t

View 3i3t on RCSB PDB site
Description: Crystal structure of covalent ubiquitin-USP21 complex
Class: hydrolase
Keywords: ubiquitin-specific protease activity, HYDROLASE, Ubiquitin biology, Structural Genomics Consortium, SGC, Activator, Chromatin regulator, Nucleus, Protease, Thiol protease, Transcription, Transcription regulation, Ubl conjugation pathway, Isopeptide bond, Phosphoprotein
Deposited on 2009-06-30, released 2009-07-21
The last revision prior to the SCOPe 2.04 freeze date was dated 2013-02-27, with a file datestamp of 2013-02-22.
Experiment type: XRAY
Resolution: 2.59 Å
R-factor: 0.188
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ubiquitin carboxyl-terminal hydrolase 21
    Species: Homo sapiens [TaxId:9606]
    Gene: PP1490, USP21, USP23
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3ta_
  • Chain 'B':
    Compound: Ubiquitin
    Species: Homo sapiens [TaxId:9606]
    Gene: RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBQ
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3tb_
  • Chain 'C':
    Compound: Ubiquitin carboxyl-terminal hydrolase 21
    Species: Homo sapiens [TaxId:9606]
    Gene: PP1490, USP21, USP23
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3tc_
  • Chain 'D':
    Compound: Ubiquitin
    Species: Homo sapiens [TaxId:9606]
    Gene: RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBQ
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3td_
  • Chain 'E':
    Compound: Ubiquitin carboxyl-terminal hydrolase 21
    Species: Homo sapiens [TaxId:9606]
    Gene: PP1490, USP21, USP23
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3te_
  • Chain 'F':
    Compound: Ubiquitin
    Species: Homo sapiens [TaxId:9606]
    Gene: RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBQ
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3tf_
  • Chain 'G':
    Compound: Ubiquitin carboxyl-terminal hydrolase 21
    Species: Homo sapiens [TaxId:9606]
    Gene: PP1490, USP21, USP23
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3tg_
  • Chain 'H':
    Compound: Ubiquitin
    Species: Homo sapiens [TaxId:9606]
    Gene: RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBQ
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3i3th_
  • Heterogens: ZN, NEH, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3i3tA (A:)
    sghvglrnlgntcflnavlqclsstrplrdfclrrdfrqevpgggraqelteafadviga
    lwhpdsceavnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrgrrappil
    angpvpspprrggalleepelsdddranlmwkryleredskivdlfvgqlksclkcqacg
    yrsttfevfcdlslpipkkgfaggkvslrdcfnlftkeeelesenapvcdrcrqktrstk
    kltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslgdfasdkagspvyqlyalc
    nhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlfyqlmqeppr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3i3tA (A:)
    hvglrnlgntcflnavlqclsstrplrdfclrrdfrqevqelteafadvigalwhpdsce
    avnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrlsdddranlmwkryle
    redskivdlfvgqlksclkcqacgyrsttfevfcdlslpipkvslrdcfnlftkeeeles
    enapvcdrcrqktrstkkltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslgd
    fasspvyqlyalcnhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlfy
    qlm
    

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3i3tB (B:)
    mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
    iqkestlhlvlrlrg
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3i3tC (C:)
    sghvglrnlgntcflnavlqclsstrplrdfclrrdfrqevpgggraqelteafadviga
    lwhpdsceavnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrgrrappil
    angpvpspprrggalleepelsdddranlmwkryleredskivdlfvgqlksclkcqacg
    yrsttfevfcdlslpipkkgfaggkvslrdcfnlftkeeelesenapvcdrcrqktrstk
    kltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslgdfasdkagspvyqlyalc
    nhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlfyqlmqeppr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3i3tC (C:)
    hvglrnlgntcflnavlqclsstrplrdfclrrdfrqevqelteafadvigalwhpdsce
    avnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrlsdddranlmwkryle
    redskivdlfvgqlksclkcqacgyrsttfevfcdlslpipkkgfaggkvslrdcfnlft
    keeelesenapvcdrcrqktrstkkltvqrfprilvlhlnrfsasrgsikkssvgvdfpl
    qrlslgdfasspvyqlyalcnhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvass
    egyvlfyqlm
    

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3i3tD (D:)
    mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
    iqkestlhlvlrlrg
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >3i3tE (E:)
    sghvglrnlgntcflnavlqclsstrplrdfclrrdfrqevpgggraqelteafadviga
    lwhpdsceavnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrgrrappil
    angpvpspprrggalleepelsdddranlmwkryleredskivdlfvgqlksclkcqacg
    yrsttfevfcdlslpipkkgfaggkvslrdcfnlftkeeelesenapvcdrcrqktrstk
    kltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslgdfasdkagspvyqlyalc
    nhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlfyqlmqeppr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3i3tE (E:)
    hvglrnlgntcflnavlqclsstrplrdfclrrdfrqevqelteafadvigalwhpdsce
    avnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrlsdddranlmwkryle
    redskivdlfvgqlksclkcqacgyrsttfevfcdlslpipkkgfaggkvslrdcfnlft
    keeelesenapvcdrcrqktrstkkltvqrfprilvlhlnrfsasrgsikkssvgvdfpl
    qrlslgdfasspvyqlyalcnhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvass
    egyvlfyqlm
    

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3i3tF (F:)
    mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
    iqkestlhlvlrlrg
    

  • Chain 'G':
    Sequence, based on SEQRES records: (download)
    >3i3tG (G:)
    sghvglrnlgntcflnavlqclsstrplrdfclrrdfrqevpgggraqelteafadviga
    lwhpdsceavnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrgrrappil
    angpvpspprrggalleepelsdddranlmwkryleredskivdlfvgqlksclkcqacg
    yrsttfevfcdlslpipkkgfaggkvslrdcfnlftkeeelesenapvcdrcrqktrstk
    kltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslgdfasdkagspvyqlyalc
    nhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlfyqlmqeppr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3i3tG (G:)
    hvglrnlgntcflnavlqclsstrplrdfclrrdfrqevqelteafadvigalwhpdsce
    avnptrfravfqkyvpsfsgysqqdaqeflkllmerlhleinrrlsdddranlmwkryle
    redskivdlfvgqlksclkcqacgyrsttfevfcdlslpipkkvslrdcfnlftkeeele
    senapvcdrcrqktrstkkltvqrfprilvlhlnrfsasrgsikkssvgvdfplqrlslg
    dfasspvyqlyalcnhsgsvhyghytalcrcqtgwhvyndsrvspvsenqvassegyvlf
    yqlm
    

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3i3tH (H:)
    mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
    iqkestlhlvlrlrg