SCOPe 2.04: Structural Classification of Proteins

PDB entry 3i3d

View 3i3d on RCSB PDB site
Description: E. COLI (lacZ) BETA-GALACTOSIDASE (M542A) IN COMPLEX WITH IPTG
Class: hydrolase
Keywords: BETA-GALACTOSIDASE, TIM BARREL (ALPHA/BETA BARREL), JELLY-ROLL BARREL, IMMUNOGLOBULIN BETA SUPERSANDWHICH, Glycosidase, Hydrolase
Deposited on 2009-06-30, released 2010-05-12
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-08-10, with a file datestamp of 2011-08-05.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.185
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: beta-galactosidase
Species: Escherichia coli [TaxId:83333]
Gene: lacZ
Database cross-references and differences (RAF-indexed):
- Uniprot B8LFD6 (Start-1022)
  - engineered (541)
Domains in SCOPe 2.04: d3i3da1, d3i3da2, d3i3da3, d3i3da4, d3i3da5
Chain 'B':
Compound: beta-galactosidase
Species: Escherichia coli [TaxId:83333]
Gene: lacZ
Database cross-references and differences (RAF-indexed):
- Uniprot B8LFD6 (Start-1022)
  - engineered (541)
Domains in SCOPe 2.04: d3i3db1, d3i3db2, d3i3db3, d3i3db4, d3i3db5
Chain 'C':
Compound: beta-galactosidase
Species: Escherichia coli [TaxId:83333]
Gene: lacZ
Database cross-references and differences (RAF-indexed):
- Uniprot B8LFD6 (Start-1022)
  - engineered (541)
Domains in SCOPe 2.04: d3i3dc1, d3i3dc2, d3i3dc3, d3i3dc4, d3i3dc5
Chain 'D':
Compound: beta-galactosidase
Species: Escherichia coli [TaxId:83333]
Gene: lacZ
Database cross-references and differences (RAF-indexed):
- Uniprot B8LFD6 (Start-1022)
  - engineered (541)
Domains in SCOPe 2.04: d3i3dd1, d3i3dd2, d3i3dd3, d3i3dd4, d3i3dd5
Heterogens: IPT, MG, NA, DMS, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>3i3dA (A:)
gshmledpvvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrf
awfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenpt
gcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflrag
enrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravl
eaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpkl
wsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehh
plhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgm
vpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdp
srpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyah
aagnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrq
fcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldg
kplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqw
rlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltpl
rdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittah
awqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlgl
gpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnis
rysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvw
cqk

Sequence, based on observed residues (ATOM records): (download)

>3i3dA (A:)
rrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfawfpapeavpes
wlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgcysltfnvdes
wlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragenrlavmvlrws
dgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravleaevqmcgelrd
ylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklwsaeipnlyrav
velhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhplhgqvmdeqtm
vqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvpmnrltddprw
lpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsrpvqyegggad
ttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyahaagnslggfaky
wqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfcmnglvfadrt
phpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkplasgevpldv
apqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrlaenlsvtlpa
ashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrapldnd
igvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgktlfis
rktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypdrlta
acfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlmetsh
rhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk

Chain 'B':
Sequence, based on SEQRES records: (download)

>3i3dB (B:)
gshmledpvvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrf
awfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenpt
gcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflrag
enrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravl
eaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpkl
wsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehh
plhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgm
vpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdp
srpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyah
aagnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrq
fcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldg
kplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqw
rlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltpl
rdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittah
awqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlgl
gpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnis
rysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvw
cqk

Sequence, based on observed residues (ATOM records): (download)

>3i3dB (B:)
rrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfawfpapeavpes
wlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgcysltfnvdes
wlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragenrlavmvlrws
dgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravleaevqmcgelrd
ylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklwsaeipnlyrav
velhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhplhgqvmdeqtm
vqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvpmnrltddprw
lpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsrpvqyegggad
ttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyahaagnslggfaky
wqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfcmnglvfadrt
phpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkplasgevpldv
apqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrlaenlsvtlpa
ashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrapldnd
igvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgktlfis
rktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypdrlta
acfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlmetsh
rhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk

Chain 'C':
Sequence, based on SEQRES records: (download)

>3i3dC (C:)
gshmledpvvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrf
awfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenpt
gcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflrag
enrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravl
eaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpkl
wsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehh
plhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgm
vpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdp
srpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyah
aagnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrq
fcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldg
kplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqw
rlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltpl
rdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittah
awqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlgl
gpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnis
rysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvw
cqk

Sequence, based on observed residues (ATOM records): (download)

>3i3dC (C:)
rrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfawfpapeavpes
wlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgcysltfnvdes
wlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragenrlavmvlrws
dgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravleaevqmcgelrd
ylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklwsaeipnlyrav
velhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhplhgqvmdeqtm
vqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvpmnrltddprw
lpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsrpvqyegggad
ttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyahaagnslggfaky
wqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfcmnglvfadrt
phpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkplasgevpldv
apqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrlaenlsvtlpa
ashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrapldnd
igvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgktlfis
rktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypdrlta
acfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlmetsh
rhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk

Chain 'D':
Sequence, based on SEQRES records: (download)

>3i3dD (D:)
gshmledpvvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrf
awfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenpt
gcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflrag
enrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravl
eaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpkl
wsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehh
plhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgm
vpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdp
srpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyah
aagnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrq
fcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldg
kplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqw
rlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltpl
rdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittah
awqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlgl
gpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnis
rysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvw
cqk

Sequence, based on observed residues (ATOM records): (download)

>3i3dD (D:)
rrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfawfpapeavpes
wlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgcysltfnvdes
wlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragenrlavmvlrws
dgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravleaevqmcgelrd
ylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklwsaeipnlyrav
velhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhplhgqvmdeqtm
vqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvpmnrltddprw
lpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsrpvqyegggad
ttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilceyahaagnslggfaky
wqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfcmnglvfadrt
phpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkplasgevpldv
apqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrlaenlsvtlpa
ashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrapldnd
igvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgktlfis
rktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypdrlta
acfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlmetsh
rhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk