PDB entry 3i22

View 3i22 on RCSB PDB site
Description: Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
Deposited on 2009-06-28, released 2009-09-01
The last revision was dated 2014-12-10, with a file datestamp of 2014-12-05.
Experiment type: XRAY
Resolution: 3.71 Å
R-factor: 0.228
AEROSPACI score: 0.06 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 50S ribosomal protein L32
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain '1':
    Compound: 50S ribosomal protein L33
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain '2':
    Compound: 50S ribosomal protein L34
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain '3':
    Compound: 50S ribosomal protein L35
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain '4':
    Compound: 50S ribosomal protein L36
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'A':
    Compound: 23s rRNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'B':
    Compound: 5s rRNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'C':
    Compound: 50S ribosomal protein L2
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: 50S ribosomal protein L3
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: 50S ribosomal protein L4
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: 50S ribosomal protein L5
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: 50S ribosomal protein L6
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: 50S ribosomal protein L9
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: 50S ribosomal protein L11
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: 50S ribosomal protein L13
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: 50S ribosomal protein L14
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: 50S ribosomal protein L15
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: 50S ribosomal protein L16
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: 50S ribosomal protein L17
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: 50S ribosomal protein L18
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'P':
    Compound: 50S ribosomal protein L19
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Q':
    Compound: 50S ribosomal protein L20
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: 50S ribosomal protein L21
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'S':
    Compound: 50S ribosomal protein L22
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'T':
    Compound: 50S ribosomal protein L23
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'U':
    Compound: 50S ribosomal protein L24
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'V':
    Compound: 50S ribosomal protein L25
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'W':
    Compound: 50S ribosomal protein L27
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'X':
    Compound: 50S ribosomal protein L28
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Y':
    Compound: 50S ribosomal protein L29
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Z':
    Compound: 50S ribosomal protein L30
    Species: Escherichia coli K-12 [TaxId:83333]
    Database cross-references and differences (RAF-indexed):
  • Heterogens: MG, ZN, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain '0':
    Sequence, based on SEQRES records:
    >3i220 (0:)
mavqqnkptrskrgmrrshdaltavtslsvdktsgekhlrhhitadgyyrgrkviak

    Sequence, based on observed residues (ATOM records):
    >3i220 (0:)
avqqnkptrskrgmrrshdaltavtslsvdktsgekhlrhhitadgyyrgrkviak

  • Chain '1':
    Sequence, based on SEQRES records:
    >3i221 (1:)
makgirekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeakik

    Sequence, based on observed residues (ATOM records):
    >3i221 (1:)
girekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeak

  • Chain '2':
    Sequence; same for both SEQRES and ATOM records:
    >3i222 (2:)
mkrtfqpsvlkrnrshgfrarmatkngrqvlarrrakgrarltvsk

  • Chain '3':
    Sequence, based on SEQRES records:
    >3i223 (3:)
mpkiktvrgaakrfkktgkggfkhkhanlrhiltkkatkrkrhlrpkamvskgdlglvia
clpya

    Sequence, based on observed residues (ATOM records):
    >3i223 (3:)
pkiktvrgaakrfkktgkggfkhkhanlrhiltkkatkrkrhlrpkamvskgdlglviac
lpya

  • Chain '4':
    Sequence; same for both SEQRES and ATOM records:
    >3i224 (4:)
mkvrasvkklcrnckivkrdgvirvicsaepkhkqrqg

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3i22C (C:)
mavvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkq
ayrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgv
daaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsge
mrkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnf
gkhpvtpwgvqtkgkktrsnkrtdkfivrrrsk

    Sequence, based on observed residues (ATOM records):
    >3i22C (C:)
avvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkqa
yrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgvd
aaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsgem
rkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnfg
khpvtpwgvqtkgkktrsnkrtdkfivrrrs

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records:
    >3i22D (D:)
miglvgkkvgmtriftedgvsipvtvieveanrvtqvkdlandgyraiqvttgakkanrv
tkpeaghfakagveagrglwefrlaegeeftvgqsisvelfadvkkvdvtgtskgkgfag
tvkrwnfrtqdathgnslshrvpgsigqnqtpgkvfkgkkmagqmgnervtvqsldvvrv
daernlllvkgavpgatgsdlivkpavka

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records:
    >3i22E (E:)
melvlkdaqsaltvsettfgrdfnealvhqvvvayaagarqgtraqktraevtgsgkkpw
rqkgtgrarsgsikspiwrsggvtfaarpqdhsqkvnkkmyrgalksilselvrqdrliv
vekfsveapktkllaqklkdmaledvliitgeldenlflaarnlhkvdvrdatgidpvsl
iafdkvvmtadavkqveemla

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3i22F (F:)
maklhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaai
sgqkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsak
sfdgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk

    Sequence, based on observed residues (ATOM records):
    >3i22F (F:)
aklhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaais
gqkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsaks
fdgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk

  • Chain 'G':
    Sequence, based on SEQRES records:
    >3i22G (G:)
msrvakapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyad
gwaqagtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpag
itaecptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtkeakkk

    Sequence, based on observed residues (ATOM records):
    >3i22G (G:)
srvakapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyadg
waqagtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpagi
taecptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtkeakkk

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records:
    >3i22H (H:)
mqvilldkvanlgslgdqvnvkagyarnflvpqgkavpatkknieffearraeleaklae
vlaaanaraekinaletvtiaskagdegklfgsigtrdiadavtaagvevaksevrlpng
vlrttgehevsfqvhsevfakvivnvvae

  • Chain 'I':
    Sequence, based on SEQRES records:
    >3i22I (I:)
makkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvit
vyadrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtga
dieamtrsiegtarsmglvved

    Sequence, based on observed residues (ATOM records):
    >3i22I (I:)
akkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvitv
yadrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtgad
ieamtrsiegtarsmglvved

  • Chain 'J':
    Sequence; same for both SEQRES and ATOM records:
    >3i22J (J:)
mktftakpetvkrdwyvvdatgktlgrlatelarrlrgkhkaeytphvdtgdyiivlnad
kvavtgnkrtdkvyyhhtghiggikqatfeemiarrpervieiavkgmlpkgplgramfr
klkvyagnehnhaaqqpqvldi

  • Chain 'K':
    Sequence, based on SEQRES records:
    >3i22K (K:)
miqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlka
vvvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislap
evl

    Sequence, based on observed residues (ATOM records):
    >3i22K (K:)
miqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlka
vvvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislap
ev

  • Chain 'L':
    Sequence, based on SEQRES records:
    >3i22L (L:)
mrlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrr
lpkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpv
tvrglrvtkgaraaieaaggkiee

    Sequence, based on observed residues (ATOM records):
    >3i22L (L:)
rlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrrl
pkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpvt
vrglrvtkgaraaieaaggkiee

  • Chain 'M':
    Sequence; same for both SEQRES and ATOM records:
    >3i22M (M:)
mlqpkrtkfrkmhkgrnrglaqgtdvsfgsfglkavgrgrltarqieaarramtravkrq
gkiwirvfpdkpitekplavrmgkgkgnveywvaliqpgkvlyemdgvpeelareafkla
aaklpikttfvtktvm

  • Chain 'N':
    Sequence, based on SEQRES records:
    >3i22N (N:)
mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse
kaeaaae

    Sequence, based on observed residues (ATOM records):
    >3i22N (N:)
mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse

  • Chain 'O':
    Sequence, based on SEQRES records:
    >3i22O (O:)
mdkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiae
qlkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf

    Sequence, based on observed residues (ATOM records):
    >3i22O (O:)
dkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiaeq
lkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf

  • Chain 'P':
    Sequence, based on SEQRES records:
    >3i22P (P:)
msniikqleqeqmkqdvpsfrpgdtvevkvwvvegskkrlqafegvviairnrglhsaft
vrkisngegvervfqthspvvdsisvkrrgavrkaklyylrertgkaarikerln

    Sequence, based on observed residues (ATOM records):
    >3i22P (P:)
sniikqleqeqmkqdvpsfrpgdtvevkvwvvegskkrlqafegvviairnrglhsaftv
rkisngegvervfqthspvvdsisvkrrgavrkaklyylrertgkaarikerln

  • Chain 'Q':
    Sequence, based on SEQRES records:
    >3i22Q (Q:)
marvkrgviararhkkilkqakgyygarsrvyrvafqavikagqyayrdrrqrkrqfrql
wiarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftalvekakaala

    Sequence, based on observed residues (ATOM records):
    >3i22Q (Q:)
arvkrgviararhkkilkqakgyygarsrvyrvafqavikagqyayrdrrqrkrqfrqlw
iarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftalvekakaala

  • Chain 'R':
    Sequence; same for both SEQRES and ATOM records:
    >3i22R (R:)
myavfqsggkqhrvsegqtvrlekldiatgetvefaevlmiangeevkigvpfvdggvik
aevvahgrgekvkivkfrrrkhyrkqqghrqwftdvkitgisa

  • Chain 'S':
    Sequence; same for both SEQRES and ATOM records:
    >3i22S (S:)
metiakhrharssaqkvrlvadlirgkkvsqaldiltytnkkaavlvkkvlesaianaeh
ndgadiddlkvtkifvdegpsmkrimprakgradrilkrtshitvvvsdr

  • Chain 'T':
    Sequence, based on SEQRES records:
    >3i22T (T:)
mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
lvvkgkvkrhgqrigrrsdwkkayvtlkegqnldfvggae

    Sequence, based on observed residues (ATOM records):
    >3i22T (T:)
mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
lvvkgkvkrhgqrigrrsdwkkayvtlkegqnl

  • Chain 'U':
    Sequence, based on SEQRES records:
    >3i22U (U:)
maakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggive
keaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnsetik

    Sequence, based on observed residues (ATOM records):
    >3i22U (U:)
aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnseti

  • Chain 'V':
    Sequence; same for both SEQRES and ATOM records:
    >3i22V (V:)
mftinaevrkeqgkgasrrlraankfpaiiyggkeaplaieldhdkvmnmqakaefysev
ltivvdgkeikvkaqdvqrhpykpklqhidfvra

  • Chain 'W':
    Sequence, based on SEQRES records:
    >3i22W (W:)
mahkkaggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlf
akadgkvkfevkgpknrkfisieae

    Sequence, based on observed residues (ATOM records):
    >3i22W (W:)
ggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfakadgk
vkfevkgpknrkfisieae

  • Chain 'X':
    Sequence, based on SEQRES records:
    >3i22X (X:)
msrvcqvtgkrpvtgnnrshalnatkrrflpnlhshrfwvesekrfvtlrvsakgmrvid
kkgidtvlaelrargeky

    Sequence, based on observed residues (ATOM records):
    >3i22X (X:)
srvcqvtgkrpvtgnnrshalnatkrrflpnlhshrfwvesekrfvtlrvsakgmrvidk
kgidtvlaelrargeky

  • Chain 'Y':
    Sequence; same for both SEQRES and ATOM records:
    >3i22Y (Y:)
mkakelreksveelntellnllreqfnlrmqaasgqlqqshllkqvrrdvarvktllnek
aga

  • Chain 'Z':
    Sequence, based on SEQRES records:
    >3i22Z (Z:)
maktikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee

    Sequence, based on observed residues (ATOM records):
    >3i22Z (Z:)
aktikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee