PDB entry 3hj3

View 3hj3 on RCSB PDB site
Description: Crystal Structure of the ChTS-DHFR F207A Non-Active Site Mutant
Class: oxidoreductase
Keywords: ts, dhfr, enzyme, crossover, non-active site, oxidoreductase
Deposited on 2009-05-20, released 2010-06-02
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-01-15, with a file datestamp of 2014-01-10.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.228
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (Start-520)
      • engineered (206)
    Domains in SCOPe 2.07: d3hj3a1, d3hj3a2
  • Chain 'B':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (Start-520)
      • engineered (206)
    Domains in SCOPe 2.07: d3hj3b1, d3hj3b2
  • Chain 'C':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (Start-520)
      • engineered (206)
    Domains in SCOPe 2.07: d3hj3c1, d3hj3c2
  • Chain 'D':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (Start-520)
      • engineered (206)
    Domains in SCOPe 2.07: d3hj3d1, d3hj3d2
  • Heterogens: UMP, CB3, MTX, NDP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3hj3A (A:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >3hj3A (A:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekql
ksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengay
renrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfikgdtngnhliekkv
yiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklie
tlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspf
niasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkv
eniedfkwedieligyypyptikmdmav

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3hj3B (B:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >3hj3B (B:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekrg
qlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvleng
ayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfikgdtngnhliek
kvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlakl
ietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgs
pfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkr
kveniedfkwedieligyypyptikmdmav

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3hj3C (C:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >3hj3C (C:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
qlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvleng
ayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfikgdtngnhliek
kvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlakl
ietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgs
pfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkr
kveniedfkwedieligyypyptikmdmav

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3hj3D (D:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairsifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >3hj3D (D:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqlksi
ddtvdllgeiagirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengayren
rtgistysifgqmmrfdmresfpllttkkvairsifeeliwfikgdtngnhliekkvyiw
sgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklietlk
nnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspfnia
syailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkveni
edfkwedieligyypyptikmdmav