PDB entry 3hej

View 3hej on RCSB PDB site
Description: Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature
Class: hydrolase
Keywords: Staphylococcal nuclease, hyperstable variant, hydrolase, pdtp, domain swapping, arginine, Calcium, Endonuclease, Membrane, Metal-binding, Nuclease, Secreted, Zymogen
Deposited on 2009-05-08, released 2010-05-19
The last revision prior to the SCOPe 2.05 freeze date was dated 2010-05-19, with a file datestamp of 2010-05-14.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.207
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thermonuclease
    Species: Staphylococcus aureus [TaxId:1280]
    Gene: nuc
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00644 (0-End)
      • engineered (43-44)
      • engineered (55)
      • engineered (110)
      • engineered (117)
      • engineered (121)
    Domains in SCOPe 2.05: d3heja_
  • Chain 'B':
    Compound: Thermonuclease
    Species: Staphylococcus aureus [TaxId:1280]
    Gene: nuc
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00644 (0-End)
      • engineered (43-44)
      • engineered (55)
      • engineered (110)
      • engineered (117)
      • engineered (121)
    Domains in SCOPe 2.05: d3hejb_
  • Chain 'C':
    Compound: Thermonuclease
    Species: Staphylococcus aureus [TaxId:1280]
    Gene: nuc
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00644 (0-End)
      • engineered (43-44)
      • engineered (55)
      • engineered (110)
      • engineered (117)
      • engineered (121)
    Domains in SCOPe 2.05: d3hejc_
  • Chain 'D':
    Compound: Thermonuclease
    Species: Staphylococcus aureus [TaxId:1280]
    Gene: nuc
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00644 (0-End)
      • engineered (43-44)
      • engineered (55)
      • engineered (110)
      • engineered (117)
      • engineered (121)
    Domains in SCOPe 2.05: d3hejd_
  • Heterogens: THP, MPD, GOL, MRD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3hejA (A:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq

    Sequence, based on observed residues (ATOM records): (download) 
    >3hejA (A:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniws

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3hejB (B:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq

    Sequence, based on observed residues (ATOM records): (download) 
    >3hejB (B:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniws

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3hejC (C:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq

    Sequence, based on observed residues (ATOM records): (download) 
    >3hejC (C:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniws

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3hejD (D:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq

    Sequence, based on observed residues (ATOM records): (download) 
    >3hejD (D:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafrkkmv
enakkievefdkgqrtdkygrglayiyadgkmvnealvrqglakvayvykgnntheqllr
kaeaqakkeklniws