PDB entry 3h99

View 3h99 on RCSB PDB site
Description: structure of a mutant methionyl-trna synthetase with modified specificity complexed with methionine
Deposited on 2009-04-30, released 2009-12-08
The last revision was dated 2021-10-13, with a file datestamp of 2021-10-08.
Experiment type: XRAY
Resolution: 1.4 Å
R-factor: 0.168
AEROSPACI score: 0.7 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Methionyl-tRNA synthetase
    Species: Escherichia coli [TaxId:83333]
    Gene: b2114, JW2101, metG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00959 (12-559)
      • expression tag (0-11)
      • engineered mutation (25)
      • engineered mutation (272)
      • engineered mutation (313)
  • Heterogens: MET, ZN, CIT, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3h99A (A:)
    mrgshhhhhhgsmtqvakkilvtcaspyangsihlghmlehiqadvwvryqrmrghevnf
    icaddahgtpimlkaqqlgitpeqmigemsqehqtdfagfnisydnyhsthseenrqlse
    liysrlkengfiknrtisqlydpekgmflpdrfvkgtcpkckspdqygdncevcgatysp
    teliepksvvsgatpvmrdsehfffdlpsfsemlqawtrsgalqeqvankmqewfesglq
    qwdisrdapyfgfeipnapgkyfyvwldapiglmgsfknlcdkrgdsvsfdeywkkdsta
    elyhfigkdivyflslfwpamlegsnfrkpsnlfvhgyvtvngakmsksrgtfikastwl
    nhfdadslryyytaklssriddidlnledfvqrvnadivnkvvnlasrnagfinkrfdgv
    laseladpqlyktftdaaevigeawesrefgkavreimaladlanryvdeqapwvvakqe
    grdadlqaicsmginlfrvlmtylkpvlpklteraeaflnteltwdgiqqpllghkvnpf
    kalynridmrqvealveask
    

    Sequence, based on observed residues (ATOM records):
    >3h99A (A:)
    akkilvtcaspyangsihlghmlehiqadvwvryqrmrghevnficaddahgtpimlkaq
    qlgitpeqmigemsqehqtdfagfnisydnyhsthseenrqlseliysrlkengfiknrt
    isqlydpekgmflpdrfvkgtcpkckspdqygdncevcgatyspteliepksvvsgatpv
    mrdsehfffdlpsfsemlqawtrsgalqeqvankmqewfesglqqwdisrdapyfgfeip
    napgkyfyvwldapiglmgsfknlcdkrgdsvsfdeywkkdstaelyhfigkdivyflsl
    fwpamlegsnfrkpsnlfvhgyvtvngakmsksrgtfikastwlnhfdadslryyytakl
    ssriddidlnledfvqrvnadivnkvvnlasrnagfinkrfdgvlaseladpqlyktftd
    aaevigeawesrefgkavreimaladlanryvdeqapwvvakqegrdadlqaicsmginl
    frvlmtylkpvlpklteraeaflnteltwdgiqqpllghkvnpfkalynridmrqvealv
    eask