PDB entry 3h4h

View 3h4h on RCSB PDB site
Description: Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis
Class: oxidoreductase
Keywords: nitrite reductase, high-throuhput screening, oxidase, copper, FAD, Flavoprotein, Metal-binding, Nitrate assimilation, Oxidoreductase, Periplasm, Pyrrolidone carboxylic acid
Deposited on 2009-04-20, released 2010-02-02
The last revision prior to the SCOPe 2.08 freeze date was dated 2010-02-16, with a file datestamp of 2010-02-12.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: 0.183
AEROSPACI score: 0.59 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Copper-containing nitrite reductase
    Species: Alcaligenes faecalis [TaxId:511]
    Gene: nir, nirK
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38501 (0-334)
      • engineered (89)
      • engineered (307)
    Domains in SCOPe 2.08: d3h4ha1, d3h4ha2
  • Chain 'B':
    Compound: Copper-containing nitrite reductase
    Species: Alcaligenes faecalis [TaxId:511]
    Gene: nir, nirK
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38501 (0-334)
      • engineered (89)
      • engineered (307)
    Domains in SCOPe 2.08: d3h4hb1, d3h4hb2
  • Chain 'C':
    Compound: Copper-containing nitrite reductase
    Species: Alcaligenes faecalis [TaxId:511]
    Gene: nir, nirK
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38501 (0-334)
      • engineered (89)
      • engineered (307)
    Domains in SCOPe 2.08: d3h4hc1, d3h4hc2
  • Heterogens: CU, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3h4hA (A:)
taaeiaalprqkvelvdppfvhahsqvaeggpkvveftmvieekkividdagtevhamaf
ngtvpgplmvvhqddyleltlinpetntlthnidfhaatgalgggglteinpgektilrf
katkpgvfvyhcappgmvpwhvvsgmngaimvlpreglhdgkgkaltydkiyyvgeqdfy
vprdengkykkyeapgdayedtvkvmrtltpthvvfngavgaltgdkamtaavgekvliv
hsqanrdtrphligghgdyvwatgkfntppdvdqetwfipggaagaafytfqqpgiyayv
nhnlieacelgaaahfkvtgewnddlmtsvlapsg

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3h4hB (B:)
taaeiaalprqkvelvdppfvhahsqvaeggpkvveftmvieekkividdagtevhamaf
ngtvpgplmvvhqddyleltlinpetntlthnidfhaatgalgggglteinpgektilrf
katkpgvfvyhcappgmvpwhvvsgmngaimvlpreglhdgkgkaltydkiyyvgeqdfy
vprdengkykkyeapgdayedtvkvmrtltpthvvfngavgaltgdkamtaavgekvliv
hsqanrdtrphligghgdyvwatgkfntppdvdqetwfipggaagaafytfqqpgiyayv
nhnlieacelgaaahfkvtgewnddlmtsvlapsg

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3h4hC (C:)
taaeiaalprqkvelvdppfvhahsqvaeggpkvveftmvieekkividdagtevhamaf
ngtvpgplmvvhqddyleltlinpetntlthnidfhaatgalgggglteinpgektilrf
katkpgvfvyhcappgmvpwhvvsgmngaimvlpreglhdgkgkaltydkiyyvgeqdfy
vprdengkykkyeapgdayedtvkvmrtltpthvvfngavgaltgdkamtaavgekvliv
hsqanrdtrphligghgdyvwatgkfntppdvdqetwfipggaagaafytfqqpgiyayv
nhnlieacelgaaahfkvtgewnddlmtsvlapsg