PDB entry 3gun

View 3gun on RCSB PDB site
Description: T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--aniline binding
Class: hydrolase
Keywords: T4 lysozyme, apolar cavity, buried charge, ligand binding, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase
Deposited on 2009-03-30, released 2009-08-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-11-01, with a file datestamp of 2017-10-27.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: N/A
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lysozyme
    Species: Enterobacteria phage T4 [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (0-End)
      • engineered (20)
      • engineered (37)
      • engineered (98)
      • engineered (101)
      • engineered (107)
      • engineered (116)
      • engineered (141)
      • engineered (143)
    Domains in SCOPe 2.08: d3guna_
  • Chain 'B':
    Compound: lysozyme
    Species: Enterobacteria phage T4 [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (0-End)
      • engineered (20)
      • engineered (37)
      • engineered (98)
      • engineered (101)
      • engineered (107)
      • engineered (116)
      • engineered (141)
      • engineered (143)
    Domains in SCOPe 2.08: d3gunb_
  • Heterogens: ANL, CA, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3gunA (A:)
mnifemlrideglrlkiykdcegyytigighlltkspdlnaakseldkaigrncngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainevfqmgvtgvagftnvlrm
lqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >3gunA (A:)
mnifemlrideglrlkiykdcegyytigighlltkspdlnaakseldkaigrncngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainevfqmgvtgvagftnvlrm
lqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3gunB (B:)
mnifemlrideglrlkiykdcegyytigighlltkspdlnaakseldkaigrncngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainevfqmgvtgvagftnvlrm
lqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >3gunB (B:)
mnifemlrideglrlkiykdcegyytigighlltkspdlnaakseldkaigrncngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrrcaainevfqmgvtgvagftnvlrm
lqqkrwdeaavnlaksrwynqcpdrakrvittfrtgtwdayk