PDB entry 3glg

View 3glg on RCSB PDB site
Description: crystal structure of a mutant (gammat157a) e. coli clamp loader bound to primer-template dna
Deposited on 2009-03-12, released 2009-05-26
The last revision was dated 2021-10-20, with a file datestamp of 2021-10-15.
Experiment type: XRAY
Resolution: 3.25 Å
R-factor: 0.226
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase III subunit delta
    Species: Escherichia coli [TaxId:83333]
    Gene: holA, b0640, JW0635
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'C':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'D':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'E':
    Compound: DNA polymerase III subunit delta'
    Species: Escherichia coli [TaxId:83333]
    Gene: holB, b1099, JW1085
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: DNA polymerase III subunit delta
    Species: Escherichia coli [TaxId:83333]
    Gene: holA, b0640, JW0635
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'H':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'I':
    Compound: DNA polymerase III subunit tau
    Species: Escherichia coli [TaxId:83333]
    Gene: dnaX, dnaZ, dnaZX, b0470, JW0459
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06710 (22-394)
      • expression tag (0-21)
      • engineered mutation (178)
  • Chain 'J':
    Compound: DNA polymerase III subunit delta'
    Species: Escherichia coli [TaxId:83333]
    Gene: holB, b1099, JW1085
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: DNA (5'-d(*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*ap*tp*ap*gp*gp*cp*cp*ap*g)-3')
  • Chain 'L':
    Compound: DNA (5'-d(*cp*tp*gp*gp*cp*cp*tp*ap*tp*a)-3')
  • Chain 'M':
    Compound: DNA (5'-d(*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*ap*tp*ap*gp*gp*cp*cp*ap*g)-3')
  • Chain 'N':
    Compound: DNA (5'-d(*cp*tp*gp*gp*cp*cp*tp*ap*tp*a)-3')
  • Heterogens: ADP, BEF, MG, ZN

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3glgA (A:)
mirlypeqlraqlneglraaylllgndplllqesqdavrqvaaaqgfeehhtfsidpntd
wnaifslcqamslfasrqtlllllpengpnaaineqlltltgllhddlllivrgnklska
qenaawftalanrsvqvtcqtpeqaqlprwvaarakqlnlelddaanqvlcycyegnlla
laqalerlsllwpdgkltlprveqavndaahftpfhwvdallmgkskralhilqqlrleg
sepvillrtlqrellllvnlkrqsahtplralfdkhrvwqnrrgmmgealnrlsqtqlrq
avqlltrteltlkqdygqsvwaeleglslllchkpladvfidg

    Sequence, based on observed residues (ATOM records):
    >3glgA (A:)
mirlypeqlraqlneglraaylllgndplllqesqdavrqvaaaqgfeehhtfsidpntd
wnaifslcqamslfasrqtlllllpengpnaaineqlltltgllhddlllivrgnklska
qenaawftalanrsvqvtcqtpeqaqlprwvaarakqlnlelddaanqvlcycyegnlla
laqalerlsllwpdgkltlprveqavndaahftpfhwvdallmgkskralhilqqlrleg
sepvillrtlqrellllvnlkrqsahtplralfdkhrvwqnrrgmmgealnrlsqtqlrq
avqlltrteltlkqdygqsvwaeleglslllch

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3glgB (B:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgB (B:)
vlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvgktsiarllakglnc
etgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldnvqyapargrfkvyl
idevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrclqfhlkaldveqir
hqlehilneehiahepralqllaraaegslrdalsltdqaiasgdgqvstqavsamlgtl
dddqalslveamveangervmalineaaargieweallvemlgllhriamvqlspaalgn
dmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgvemtllralafhprmp
lpep

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3glgC (C:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgC (C:)
qvlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvgktsiarllakgln
cetgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldnvqyapargrfkvy
lidevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrclqfhlkaldveqi
rhqlehilneehiahepralqllaraaegslrdalsltdqaiasgdgqvstqavsamlgt
ldddqalslveamveangervmalineaaargieweallvemlgllhriamvqlspaalg
ndmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgvemtllralafhprm
plpep

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3glgD (D:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgD (D:)
syqvlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvgktsiarllakg
lncetgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldnvqyapargrfk
vylidevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrclqfhlkaldve
qirhqlehilneehiahepralqllaraaegslrdalsltdqaiasgdgqvstqavsaml
gtldddqalslveamveangervmalineaaargieweallvemlgllhriamvqlspaa
lgndmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgvemtllralafhp
rm

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records:
    >3glgE (E:)
mrwypwlrpdfeklvasyqagrghhalliqalpgmgddaliyalsryllcqqpqghkscg
hcrgcqlmqagthpdyytlapekgkntlgvdavrevteklneharlggakvvwvtdaall
tdaaanallktleeppaetwfflatreperllatlrsrcrlhylapppeqyavtwlsrev
tmsqdallaalrlsagspgaalalfqgdnwqaretlcqalaysvpsgdwysllaalnheq
aparlhwlatllmdalkrhhgaaqvtnvdvpglvaelanhlspsrlqailgdvchireql
msvtginrellitdlllriehylqpgvvlpvphl

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3glgF (F:)
mirlypeqlraqlneglraaylllgndplllqesqdavrqvaaaqgfeehhtfsidpntd
wnaifslcqamslfasrqtlllllpengpnaaineqlltltgllhddlllivrgnklska
qenaawftalanrsvqvtcqtpeqaqlprwvaarakqlnlelddaanqvlcycyegnlla
laqalerlsllwpdgkltlprveqavndaahftpfhwvdallmgkskralhilqqlrleg
sepvillrtlqrellllvnlkrqsahtplralfdkhrvwqnrrgmmgealnrlsqtqlrq
avqlltrteltlkqdygqsvwaeleglslllchkpladvfidg

    Sequence, based on observed residues (ATOM records):
    >3glgF (F:)
mirlypeqlraqlneglraaylllgndplllqesqdavrqvaaaqgfeehhtfsidpntd
wnaifslcqamslfasrqtlllllpengpnaaineqlltltgllhddlllivrgnklska
qenaawftalanrsvqvtcqtpeqaqlprwvaarakqlnlelddaanqvlcycyegnlla
laqalerlsllwpdgkltlprveqavndaahftpfhwvdallmgkskralhilqqlrleg
sepvillrtlqrellllvnlkrqsahtplralfdkhrvwqnrrgmmgealnrlsqtqlrq
avqlltrteltlkqdygqsvwaeleglslllch

  • Chain 'G':
    Sequence, based on SEQRES records:
    >3glgG (G:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgG (G:)
glevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvg
ktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldn
vqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrc
lqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdalsltdqaiasgdg
qvstqavsamlgtldddqalslveamveangervmalineaaargieweallvemlgllh
riamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgve
mtllralafhprmplpep

  • Chain 'H':
    Sequence, based on SEQRES records:
    >3glgH (H:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgH (H:)
qvlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvgktsiarllakgln
cetgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldnvqyapargrfkvy
lidevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrclqfhlkaldveqi
rhqlehilneehiahepralqllaraaegslrdalsltdqaiasgdgqvstqavsamlgt
ldddqalslveamveangervmalineaaargieweallvemlgllhriamvqlspaalg
ndmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgvemtllralafhprm
plpep

  • Chain 'I':
    Sequence, based on SEQRES records:
    >3glgI (I:)
mgsshhhhhhssglevlfqgphmsyqvlarkwrpqtfadvvgqehvltalanglslgrih
haylfsgtrgvgktsiarllakglncetgitatpcgvcdncreieqgrfvdlieidaasr
tkvedtrdlldnvqyapargrfkvylidevhmlsrhsfnallktleeppehvkfllatad
pqklpvtilsrclqfhlkaldveqirhqlehilneehiahepralqllaraaegslrdal
sltdqaiasgdgqvstqavsamlgtldddqalslveamveangervmalineaaargiew
eallvemlgllhriamvqlspaalgndmaaielrmrelartipptdiqlyyqtlligrke
lpyapdrrmgvemtllralafhprmplpepevprq

    Sequence, based on observed residues (ATOM records):
    >3glgI (I:)
syqvlarkwrpqtfadvvgqehvltalanglslgrihhaylfsgtrgvgktsiarllakg
lncetgitatpcgvcdncreieqgrfvdlieidaasrtkvedtrdlldnvqyapargrfk
vylidevhmlsrhsfnallktleeppehvkfllatadpqklpvtilsrclqfhlkaldve
qirhqlehilneehiahepralqllaraaegslrdalsltdqaiasgdgqvstqavsaml
gtldddqalslveamveangervmalineaaargieweallvemlgllhriamvqlspaa
lgndmaaielrmrelartipptdiqlyyqtlligrkelpyapdrrmgvemtllralafhp
rm

  • Chain 'J':
    Sequence; same for both SEQRES and ATOM records:
    >3glgJ (J:)
mrwypwlrpdfeklvasyqagrghhalliqalpgmgddaliyalsryllcqqpqghkscg
hcrgcqlmqagthpdyytlapekgkntlgvdavrevteklneharlggakvvwvtdaall
tdaaanallktleeppaetwfflatreperllatlrsrcrlhylapppeqyavtwlsrev
tmsqdallaalrlsagspgaalalfqgdnwqaretlcqalaysvpsgdwysllaalnheq
aparlhwlatllmdalkrhhgaaqvtnvdvpglvaelanhlspsrlqailgdvchireql
msvtginrellitdlllriehylqpgvvlpvphl

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.

  • Chain 'M':
    No sequence available.

  • Chain 'N':
    No sequence available.