PDB entry 3gkl

View 3gkl on RCSB PDB site
Description: Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins
Class: hydrolase
Keywords: Protein-Protein complex, Structural Genomics, Israel Structural Proteomics Center, ISPC, Bacteriocin immunity, Plasmid, Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Metal-binding, Nuclease, Zinc
Deposited on 2009-03-11, released 2009-09-08
The last revision prior to the SCOPe 2.04 freeze date was dated 2009-09-08, with a file datestamp of 2009-09-04.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.241
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: colicin-e9 immunity protein
    Species: Escherichia coli [TaxId:562]
    Gene: colE7, cea
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q47112 (Start-140)
      • engineered (109)
    Domains in SCOPe 2.04: d3gkla_
  • Chain 'B':
    Compound: colicin-e9 immunity protein
    Species: Escherichia coli [TaxId:562]
    Gene: colE7, cea
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q47112 (Start-140)
      • engineered (109)
    Domains in SCOPe 2.04: d3gklb_
  • Chain 'C':
    Compound: Colicin-E7
    Species: Escherichia coli [TaxId:562]
    Gene: imm, ceiE9
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13479
      • engineered (19)
      • engineered (23)
      • engineered (26-27)
      • engineered (33)
      • engineered (36)
      • engineered (40)
      • engineered (56)
    Domains in SCOPe 2.04: d3gklc_
  • Chain 'D':
    Compound: Colicin-E7
    Species: Escherichia coli [TaxId:562]
    Gene: imm, ceiE9
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13479
      • engineered (19)
      • engineered (23)
      • engineered (26-27)
      • engineered (33)
      • engineered (36)
      • engineered (40)
      • engineered (56)
    Domains in SCOPe 2.04: d3gkld_
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3gklA (A:)
mhhhhhhsmgkrnkpgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfddf
rkkfweevskdpelskqfsrnnndrmkvgkapktrtqdvsgkrtsfelhaekpisqnggv
ydmdnisvvtpkrhidihrgk

    Sequence, based on observed residues (ATOM records): (download) 
    >3gklA (A:)
pgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfddfrkkfweevskdpel
skqfsrnnndrmkvgkapktrtqdvsgkrtsfelhaekvydmdnisvvtpkrhidihrgk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3gklB (B:)
mhhhhhhsmgkrnkpgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfddf
rkkfweevskdpelskqfsrnnndrmkvgkapktrtqdvsgkrtsfelhaekpisqnggv
ydmdnisvvtpkrhidihrgk

    Sequence, based on observed residues (ATOM records): (download) 
    >3gklB (B:)
pgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfddfrkkfweevskdpel
skqfsrnnndrmkvgkapktrtqdvsgkrtsfelhaekvydmdnisvvtpkrhidihrgk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3gklC (C:)
melkhsisdyteaeflqlvaticdadatseeeldklithfgemtehpsgsdliyypeegd
ddspsgivntvkqwraangksgfkqg

    Sequence, based on observed residues (ATOM records): (download) 
    >3gklC (C:)
khsisdyteaeflqlvaticdadatseeeldklithfgemtehpsgsdliyypeegddds
psgivntvkqwraangksgfkq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3gklD (D:)
melkhsisdyteaeflqlvaticdadatseeeldklithfgemtehpsgsdliyypeegd
ddspsgivntvkqwraangksgfkqg

    Sequence, based on observed residues (ATOM records): (download) 
    >3gklD (D:)
khsisdyteaeflqlvaticdadatseeeldklithfgemtehpsgsdliyypeegddds
psgivntvkqwraangksgfkq