PDB entry 3gfs

View 3gfs on RCSB PDB site
Description: Structure of YhdA, K109D/D137K variant
Class: oxidoreductase
Keywords: FLAVOPROTEINS, QUINONE REDUCTASE, FLAVODOXIN, OLIGOMERIZATION, Flavoprotein, FMN, NADP, Oxidoreductase
Deposited on 2009-02-27, released 2009-09-22
The last revision prior to the SCOPe 2.04 freeze date was dated 2009-09-22, with a file datestamp of 2009-09-18.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.187
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsa_
  • Chain 'B':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsb_
  • Chain 'C':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529 (0-End)
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsc_
  • Chain 'D':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsd_
  • Chain 'E':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfse_
  • Chain 'F':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsf_
  • Chain 'G':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsg_
  • Chain 'H':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsh_
  • Chain 'I':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsi_
  • Chain 'J':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsj_
  • Chain 'K':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsk_
  • Chain 'L':
    Compound: FMN-dependent NADPH-azoreductase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: azr, BSU09340, yhdA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O07529
      • engineered (108)
      • engineered (136)
    Domains in SCOPe 2.04: d3gfsl_
  • Heterogens: FMN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3gfsA (A:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsA (A:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3gfsB (B:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsB (B:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3gfsC (C:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsC (C:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3gfsD (D:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsD (D:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3gfsE (E:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsE (E:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3gfsF (F:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsF (F:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfak

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3gfsG (G:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsG (G:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >3gfsH (H:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsH (H:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfak

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >3gfsI (I:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsI (I:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >3gfsJ (J:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsJ (J:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >3gfsK (K:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsK (K:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfaka

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >3gfsL (L:)
mnmlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqr
vtkadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrt
vmrgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfakagnpgv

    Sequence, based on observed residues (ATOM records): (download) 
    >3gfsL (L:)
mlvingtprkhgrtriaasyiaalyhtdlidlsefvlpvfngeaeqsellkvqelkqrvt
kadaivllspeyhsgmsgalknaldflsseqfkykpvallavagggdgginalnnmrtvm
rgvyanvipkqlvlkpvhidvenatvaenikesikelveelsmfak