PDB entry 3g02

View 3g02 on RCSB PDB site
Description: Structure of enantioselective mutant of epoxide hydrolase from Aspergillus niger generated by directed evolution
Class: hydrolase
Keywords: EPOXIDE HYDROLASE, ALPHA/BETA HYDROLASE FOLD, enantioselective, mutant, directed evolution, Hydrolase
Deposited on 2009-01-27, released 2009-06-09
The last revision prior to the SCOPe 2.06 freeze date was dated 2009-06-09, with a file datestamp of 2009-06-05.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: 0.184
AEROSPACI score: 0.63 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: epoxide hydrolase
    Species: ASPERGILLUS NIGER [TaxId:5061]
    Gene: hyl1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9UR30 (5-End)
      • expression tag (3-4)
      • engineered (215)
      • engineered (217)
      • engineered (219)
      • engineered (249)
      • engineered (317-318)
      • engineered (329-330)
      • engineered (350)
    Domains in SCOPe 2.06: d3g02a1, d3g02a2
  • Chain 'B':
    Compound: epoxide hydrolase
    Species: ASPERGILLUS NIGER [TaxId:5061]
    Gene: hyl1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9UR30 (5-End)
      • expression tag (3-4)
      • engineered (215)
      • engineered (217)
      • engineered (219)
      • engineered (249)
      • engineered (317-318)
      • engineered (329-330)
      • engineered (350)
    Domains in SCOPe 2.06: d3g02b1, d3g02b2
  • Heterogens: FMT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3g02A (A:)
mnhkafakfpssasispnpftvsipdeqlddlktlvrlskiapptyeslqadgrfgitse
wlttmrekwlsefdwrpfearlnsfpqftteiegltihfaalfseredavpiallhgwpg
sfvefypilqlfreeytpetlpfhlvvpslpgytfssgppldkdfglmdnarvvdqlmkd
lgfgsgyiiqggdigsfvgrllgvgfdackavhlnfcnmsappegpsieslsaaekegia
rmekfmtdgyayamehstrpstighvlssspiallawigekylqwvdkplpsetilemvs
lywltesfpraihtyrewvptasapngatpyqkelyihkpfgfsffpkdlvpvprswiat
tgnlvffrdhaegghfaalerprelktdltafveqvwqkgrshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3g02A (A:)
kafakfpssasispnpftvsipdeqlddlktlvrlskiapptyeslqadgrfgitsewlt
tmrekwlsefdwrpfearlnsfpqftteiegltihfaalfseredavpiallhgwpgsfv
efypilqlfreeytpetlpfhlvvpslpgytfssgppldkdfglmdnarvvdqlmkdlgf
gsgyiiqggdigsfvgrllgvgfdackavhlnfcnmsappegpsieslsaaekegiarme
kfmtdgyayamehstrpstighvlssspiallawigekylqwvdkplpsetilemvslyw
ltesfpraihtyrewvpttpyqkelyihkpfgfsffpkdlvpvprswiattgnlvffrdh
aegghfaalerprelktdltafveqvw

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3g02B (B:)
mnhkafakfpssasispnpftvsipdeqlddlktlvrlskiapptyeslqadgrfgitse
wlttmrekwlsefdwrpfearlnsfpqftteiegltihfaalfseredavpiallhgwpg
sfvefypilqlfreeytpetlpfhlvvpslpgytfssgppldkdfglmdnarvvdqlmkd
lgfgsgyiiqggdigsfvgrllgvgfdackavhlnfcnmsappegpsieslsaaekegia
rmekfmtdgyayamehstrpstighvlssspiallawigekylqwvdkplpsetilemvs
lywltesfpraihtyrewvptasapngatpyqkelyihkpfgfsffpkdlvpvprswiat
tgnlvffrdhaegghfaalerprelktdltafveqvwqkgrshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3g02B (B:)
kafakfpssasispnpftvsipdeqlddlktlvrlskiapptyeslqadgrfgitsewlt
tmrekwlsefdwrpfearlnsfpqftteiegltihfaalfseredavpiallhgwpgsfv
efypilqlfreeytpetlpfhlvvpslpgytfssgppldkdfglmdnarvvdqlmkdlgf
gsgyiiqggdigsfvgrllgvgfdackavhlnfcnmsappslsaaekegiarmekfmtdg
yayamehstrpstighvlssspiallawigekylqwvdkplpsetilemvslywltesfp
raihtyrewvatpyqkelyihkpfgfsffpkdlvpvprswiattgnlvffrdhaegghfa
alerprelktdltafveqvw