PDB entry 3fyo

View 3fyo on RCSB PDB site
Description: Crystal structure of the triple mutant (N23C/D247E/P249A) of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis
Class: transferase
Keywords: MANNO-OCTULOSONATE, SYNTHASE, LIPOPOLYSACCHARIDE, KDOP, KDO8 KDOPS, KDO8PS, TIM BARREL, BIOSYNTHESIS, TRANSFERASE, LYASE, Lipopolysaccharide biosynthesis
Deposited on 2009-01-22, released 2009-03-24
The last revision prior to the SCOPe 2.01 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: 0.218
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    Species: Neisseria meningitidis serogroup B [TaxId:491]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-End)
      • engineered (22)
    Domains in SCOPe 2.01: d3fyoa_
  • Chain 'B':
    Compound: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    Species: Neisseria meningitidis serogroup B [TaxId:491]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-End)
      • engineered (22)
      • engineered (246)
      • engineered (248)
    Domains in SCOPe 2.01: d3fyob_
  • Chain 'C':
    Compound: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    Species: Neisseria meningitidis serogroup B [TaxId:491]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-279)
      • engineered (22)
    Domains in SCOPe 2.01: d3fyoc_
  • Chain 'D':
    Compound: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    Species: Neisseria meningitidis serogroup B [TaxId:491]
    Gene: kdsA, NMB1283
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9JZ55 (0-End)
      • engineered (22)
      • engineered (246)
      • engineered (248)
    Domains in SCOPe 2.01: d3fyod_
  • Heterogens: CL, NA, PEP, MN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3fyoA (A:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqtrdagsaasggrraqaldlalagmatrlaglfleshpd
pklakcegasalplhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3fyoA (A:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqggrraqaldlalagmatrlaglfleshalplhlledfl
irikalddliksqpi

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3fyoB (B:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqtrdagsaasggrraqaldlalagmatrlaglfleshpd
pklakcegasalplhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3fyoB (B:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqgrraqaldlalagmatrlaglfleshpegasalplhll
edflirikalddliksqpilti

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3fyoC (C:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqtrdagsaasggrraqaldlalagmatrlaglfleshpd
pklakcegasalplhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3fyoC (C:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslraqaldlalagmatrlaglfleshpalplhlledfliri
kalddliksqpiltie

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3fyoD (D:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqtrdagsaasggrraqaldlalagmatrlaglfleshpd
pklakcegasalplhlledflirikalddliksqpiltie

    Sequence, based on observed residues (ATOM records): (download) 
    >3fyoD (D:)
mdikinditlgnnspfvlfggicvlesldstlqtcahyvevtrklgipyifkasfdkanr
ssihsyrgvgleeglkifekvkaefgipvitdvhephqcqpvaevcdviqlpaflarqtd
lvvamaktgnvvnikkpqflspsqmknivekfheagngklilcergssfgydnlvvdmlg
fgvmkqtcgnlpvifdvthslqtrraqaldlalagmatrlaglfleshpcegasalplhl
ledflirikalddliksqpil