PDB entry 3fk7
View 3fk7 on RCSB PDB site
Description: Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc
Class: transcription
Keywords: Tetracycline repressor, bacterial transcription regulation, altered inducer specificity, 4-de-dimethylamino-anhydrotetracycline, Antibiotic resistance, DNA-binding, Magnesium, Metal-binding, Repressor, Transcription, Transcription regulation, Transposable element
Deposited on
2008-12-16, released
2009-11-03
The last revision prior to the SCOPe 2.05 freeze date was dated
2009-11-03, with a file datestamp of
2009-10-30.
Experiment type: XRAY
Resolution: 2.06 Å
R-factor: 0.208
AEROSPACI score: 0.41
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: tetracycline repressor protein class b from transposon tn10, tetracycline repressor protein class d
Species: Escherichia coli [TaxId:562]
Gene: tetR
Database cross-references and differences (RAF-indexed):
- Uniprot P04483 (Start-49)
- Uniprot P0ACT4 (50-End)
- engineered (63)
- engineered (134)
- engineered (137)
Domains in SCOPe 2.05: d3fk7a1, d3fk7a2 - Chain 'B':
Compound: tetracycline repressor protein class b from transposon tn10, tetracycline repressor protein class d
Species: Escherichia coli [TaxId:562]
Gene: tetR
Database cross-references and differences (RAF-indexed):
- Uniprot P04483 (Start-49)
- Uniprot P0ACT4 (50-End)
- engineered (63)
- engineered (134)
- engineered (137)
Domains in SCOPe 2.05: d3fk7b1, d3fk7b2 - Heterogens: MG, 4DM, HOH
PDB Chain Sequences:
- Chain 'A':
Sequence, based on SEQRES records: (download)
>3fk7A (A:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaveil
arhkdyslpaageswqsflrnnamsfrrallryrdgakvhlgtrpdekqydtvetqlrfm
tengfslrdglyailavihftlgavleqqehtaaltdrpaapdenlppllrealqimdsd
dgeqaflhgleslirgfevqltallqiv
Sequence, based on observed residues (ATOM records): (download)
>3fk7A (A:)
ldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaveilarh
kdyslpaageswqsflrnnamsfrrallryrdgakvhlgtrpdekqydtvetqlrfmten
gfslrdglyailavihftlgavleqqehtaaltdapdenlppllrealqimdsddgeqaf
lhgleslirgfevqltallq
- Chain 'B':
Sequence, based on SEQRES records: (download)
>3fk7B (B:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaveil
arhkdyslpaageswqsflrnnamsfrrallryrdgakvhlgtrpdekqydtvetqlrfm
tengfslrdglyailavihftlgavleqqehtaaltdrpaapdenlppllrealqimdsd
dgeqaflhgleslirgfevqltallqiv
Sequence, based on observed residues (ATOM records): (download)
>3fk7B (B:)
ldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaveilarh
kdyslpaageswqsflrnnamsfrrallryrdgakvhlgtrpdekqydtvetqlrfmten
gfslrdglyailavihftlgavleqqehtaaltdrpnlppllrealqimdsddgeqaflh
gleslirgfevqltall